We narrowed to 5,999 results for: ATS

1. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

   Plasmid

   #228252

   Purpose

   pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   SpCas9 P23H sgRNA, mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

   Plasmid

   #228253

   Purpose

   pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-SET(1-218)

   Plasmid

   #234707

   Purpose

   expression of GFP in fusion with a C-terminally truncated SET protein

   Depositor

   Lienhard Schmitz

   Article

   Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

   Insert

   SET (SET Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   expresses only the N-terminal SET part (1-218)

   Promoter

   CMV

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-EA-evoAPOBEC1max-32NLS_NG-BlastR

   Plasmid

   #232722

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   evoAPOBEC1max H47E+S48A-32NLS linker-nCas9(NG)-UGI-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   within evoAPOBEC1: H47E+S48A; within Cas9 D10A

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2T-CMV-EA-evoAPOBEC1max_NG-BlastR

   Plasmid

   #232721

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   evoAPOBEC1max H47E+S48A-nCas9(NG)-UGI-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   within evoAPOBEC1: H47E+S48A; within Cas9 D10A

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL5.0 shCavin1KDR-EGFP

   Plasmid

   #187254

   Purpose

   Lentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)

   Depositor

   Alpha Yap

   Article

   Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

   Insert

   ShRNA for Cavin1 (Cavin1 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6, 5'LTR

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM-U6-reRNA-mmDNMT1

   Plasmid

   #205635

   Purpose

   reRNA guide targeting Mus Musculus DNMT1

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-mmDNMT1 (Dnmt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK845

   Plasmid

   #219746

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   I3S, N4T, F11L, I63T, T99P, T192S, A199P

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-HaloTag-KRas4B(CT)

   Plasmid

   #214276

   Purpose

   Mammalian expression of HaloTag fused to the C-terminal tail of KRas4B

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   HaloTag7-KRas4B(174-188) (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

    Plasmid

    #178854

    Purpose

    Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Grb2: Y160E

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

    Plasmid

    #172105

    Purpose

    Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)-Gαs (GNAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gαs: C3S

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET3d-SF-DCTN1-TS

    Plasmid

    #169375

    Purpose

    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

    Depositor

    Bernd Bukau

    Article

    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

    Insert

    dynactin subunit 1 isoform 1 (DCTN1 Human)
    

    Use

    Tags

    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

    Expression

    Bacterial

    Mutation

    Point mutations: A139T, I304T, Q322R, T516A, E577…

    Promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE (hygro) mEOS2 hYAP 1-1alpha

    Plasmid

    #124155

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE (hygro)mEOS2 hYAP 1-1gamma

    Plasmid

    #124157

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABE (hygro) mEOS2 hYAP 1-1delta

    Plasmid

    #124158

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE (hygro) mEOS2 hYAP 1-2alpha

    Plasmid

    #124159

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBABE (hygro) mEOS2 hYAP 1-2beta

    Plasmid

    #124160

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE (hygro) mEOS2 hYAP 1-2gamma

    Plasmid

    #124161

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBABE (hygro) mEOS2 hYAP 1-1beta

    Plasmid

    #124156

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    mEOS2-YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDF-casBCDE(-6)

    Plasmid

    #89730

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a shorter vers…

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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