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His6-tev-G(TTR F87E)

Plasmid

#242846

Purpose

Bacterial expression of TTR

Depositor

Peter Wright

Publication

Ritsch et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

M0G, N-terminal methionine mutated to glycine, F8…

Promoter

T7

Available since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FIRESFLIP-PBCS-E670

Plasmid

#228701

Purpose

Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Publication

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6-D491A_AF

Plasmid

#148659

Purpose

Bacterial Expression of HsNot6-D491A

Depositor

Elisa Izaurralde

Publication

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot6-D491A (CNOT6 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsNot7-D40AE42A_AF

Plasmid

#148675

Purpose

Bacterial Expression of HsNot7-D40AE42A

Depositor

Elisa Izaurralde

Publication

Chen et al Nucleic Acids Res. 2021 Jun 21;49(11):64

Insert

HsNot7-D40AE42A (CNOT7 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pM1GG

Plasmid

#108200

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Publication

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101116

Purpose

Encodes dCas9(N) fused to LPAR1

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

LPAR1-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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M3939 leu2::ADE2 Disruptor Converter

Plasmid

#51672

Purpose

Yeast marker swap plasmid for converting LEU2 to ADE2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F14

Plasmid

#23063

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 deleted amino acids 3-29

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Publication

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F22

Plasmid

#23080

Depositor

Sharon Dent

Publication

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Arginine, H4 K5R.

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F24

Plasmid

#23081

Depositor

Sharon Dent

Publication

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysine 16 to Arginine, H4 K16R.

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F25

Plasmid

#23082

Depositor

Sharon Dent

Publication

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed lysine 16 to Glutamine, H4 K16…

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F26

Plasmid

#23083

Depositor

Sharon Dent

Publication

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone 4 changed Lysine 16 to glycine, H4 K16G.

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Publication

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Expression

Mammalian

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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CytERM-mAvicFP1

Plasmid

#129510

Purpose

Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

CytERM-mAvicFP1

Expression

Mammalian

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_022

Plasmid

#123684

Purpose

Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-A4 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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