We narrowed to 8,354 results for: 11
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Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyPromoterPftsH and Synthetic promoter PRhl0L0L12Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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pMZ4004
Plasmid#204401PurposeA 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsmCinR
GFP
UseSynthetic BiologyPromoterPftsH and Synthetic promoter PCin0102DAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI
Plasmid#188703PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB143
Plasmid#101679PurposeLAA-tagged mCherry under T7 promoter controlDepositorInsertLAA-tagged mCherry under T7 promoter control
UseSynthetic BiologyExpressionBacterialAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS485
Plasmid#188890PurposeWorm Cas9 expression vector, Peft-3 promoterDepositorInsertPeft-3::Cas9
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH021_SC101_pLAC_Cas12c2_Kan
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
ExpressionBacterialAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ACTB_sgRNA
Plasmid#183885PurposepX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACTB sgRNA spacer (ACTB Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-ANLN_sgRNA
Plasmid#183874PurposepX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertANLN sgRNA spacer (ANLN Human)
UseCRISPRExpressionMammalianPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172504PurposeEncodes the GwCas7x3 protein only for bacterial recombinant protein expression for purificationDepositorInsertGwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
ExpressionBacterialAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT
Plasmid#140201PurposeFungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.DepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Cloning of crrna an…TagsdLbCas12a crRNA scaffoldPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-UAP56
Plasmid#157661PurposeExpresses UAP56 in E.coli cellDepositorInsertSpliceosome RNA helicase DDX39B (DDX39B Human)
TagsGST tagExpressionBacterialMutationdeleted amino acids 1-43Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-TRE-synPCB2.0
Plasmid#139481PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTol2 transposon donor vectorExpressionMammalianPromoterTet responsive elementAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-Cx43K258stop
Plasmid#69025PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
Tagssuper folder GFPExpressionMammalianMutationnon-monomerized sfGFP fused in frame to N-terminu…PromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAND_Int2
Plasmid#60590PurposeA transcriptional AND circuit constructed by layering NOR and NOT gatesDepositorInsertAND_Int2
ExpressionBacterialPromoterPPhlFAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAND_yfp
Plasmid#60592Purposeused with pSpec to generate an AND gate without memoryDepositorInsertAND_yfp
ExpressionBacterialAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1
Plasmid#56658Purposeexpresses constitutively active human PKCi with a deletion on the PB1 domainDepositorInsertHuman PKCi (PRKCI Human)
UseLentiviralTagsV5/HisMutationA120E and deletion of PB1 domain (aa 24–98)PromoterCMVAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-NLS-ZapCmR1.1
Plasmid#59011PurposeNuclear zinc-binding FRET biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-ZapCmR1.1 genetically encoded Zn(II) sensor
TagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Chicken ArcLight S174
Plasmid#53567PurposeGenetically encoded voltage sensor ArcLight with improved kineticsDepositorInsertChicken ArcLight-S174
ExpressionMammalianMutationGg-VSP contains an R153Q mutation and an amino ac…PromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only