We narrowed to 11,437 results for: 112

1. pCS-H2B-cerulean

   Plasmid

   #53748

   Purpose

   Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS U6a gRNA

   Plasmid

   #158961

   Purpose

   gRNA backbone driven by zebrafish U6a promoter

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   zf U6a promoter
   gRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRGE32

   Plasmid

   #63159

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-DRD2

   Plasmid

   #24099

   Depositor

   Jean-Michel Arrang

   Article

   Jeanneteau et al Mol Biol Cell. 2004 Feb . 15(2):696-705.

   Insert

   DRD2 (DRD2 Human)
   

   Use

   Tags

   alpha7 nicotinic signal peptide and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIZ-turq-3xFLAG-TREX1 D18N

   Plasmid

   #164233

   Purpose

   retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient)

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and mturquoise

   Expression

   Mammalian

   Mutation

   D18N ->nuclease deficient

   Promoter

   CMV

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EYFP-hCaM

   Plasmid

   #47603

   Depositor

   Emanuel Strehler

   Article

   Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

   Insert

   CaM (CALM3 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSN837

   Plasmid

   #184825

   Purpose

   Expresses human KIF5A in mammalian cells

   Depositor

   Kyoko Chiba

   Article

   Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

   Insert

   KIF5A (KIF5A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFPI7

   Plasmid

   #83555

   Purpose

   Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   CUP1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGCP123-GFP_g1

   Plasmid

   #153518

   Purpose

   sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSL3402 (pTnsABf_Pse)

    Plasmid

    #200901

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRL-mU6-GFPGO-IRES-mScarletI-PGK-Neo

    Plasmid

    #136897

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    3xNLS on mScarlet

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Man1B1-GFP

    Plasmid

    #163646

    Purpose

    Expresses GFP tagged Man1B1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mannosidase alpha class 1B member 1 (MAN1B1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-3FLAG-RAB5A

    Plasmid

    #176488

    Purpose

    For lentiviral transduction of 3FLAG-RAB5A

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FEMP

    Plasmid

    #191973

    Purpose

    FRET-based indicator of ER-mitochondria proximity (FEMP)

    Depositor

    Luca Scorrano

    Article

    Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    AkapYFP-Tav2a-CFP-sac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-T3-CjCAS9-pA

    Plasmid

    #131543

    Purpose

    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    FLAG-SV40NLS, SV40NLS, and polyA

    Expression

    Mammalian

    Mutation

    Human codon-optimized

    Promoter

    CAG, T3

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG2IG-Hmga2

    Plasmid

    #122294

    Purpose

    Overexpression for mouse Hmga2

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Hmga2 (Hmga2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSN838

    Plasmid

    #184826

    Purpose

    Expresses human KIF5A(∆exon27) in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (∆exon27) (KIF5A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    ∆exon27 form

    Promoter

    CMV

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LYN11-FRB-CFP

    Plasmid

    #38003

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    LYN (LYN Human)
    MTOR (MTOR )
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.tgoGAP1

    Plasmid

    #78737

    Purpose

    Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    tgoGAP1
    

    Use

    Tags

    N terminal 81 amino acids of human galactosyltran…

    Expression

    Mammalian

    Mutation

    D117A/D119A/D163A in aequorin moiety

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only