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We narrowed to 11,646 results for: emb

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Showing: 3081 - 3100 of 11646 results

  1. AP-APP

    Plasmid
    #196706
    Purpose
    Expresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    AP, Avitag and HA
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196706-plasmid-map-sequence-id-386447

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  2. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184248-plasmid-map-sequence-id-370322

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  3. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid
    #102421
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Publication
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Modified at the Dharmacon SMARTpool siRNA #2 targ…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102421-plasmid-map-sequence-id-198790

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  5. mCherry-CRY2-P14P5K

    Plasmid
    #79570
    Purpose
    expression of mCherry-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1c
    Depositor
    Olof Idevall-Hagren
    Publication
    Xie et al Cell Chem Biol. 2016 Jul 21;23(7):816-26
    Insert
    Pip5k1c (Pip5k1c Mouse)
    Tags
    CRY2 (photolyase homology region domain of Arabid…
    Expression
    Mammalian
    Mutation
    R445E and R446E; truncation of amino acids 636-66…
    Promoter
    CMV
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184249-plasmid-map-sequence-id-370367

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  11. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  12. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Publication
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    128940-plasmid-map-sequence-id-254597

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  14. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Kian Peng Koh
    Publication
    Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102422-plasmid-map-sequence-id-198789

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  18. pSems-leader-mXFPe-IFNGR2(30-337)

    Plasmid
    #192787
    Purpose
    Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Publication
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR2 (IFNGR2 Human)
    Tags
    Ig k-chain leader sequence and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192787-plasmid-map-sequence-id-379660

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  20. InterCatch-3xL

    Plasmid
    #186909
    Purpose
    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through (GGGGS)3 linker and the other side expressing EGFP and puromycin resistance gene
    Depositor
    Taekjip Ha, Allen Liu
    Publication
    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100
    Insert
    TfR-(GGGGS)3-SpyCatcher003-sfCherry1-10
    Expression
    Mammalian
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    186909-plasmid-map-sequence-id-372903

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Showing: 3081 - 3100 of 11646 results