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pUPD2 RDF ( GB1498)

Plasmid

#160579

Purpose

Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINTBxb1

Plasmid

#127519

Purpose

Plasmid encodes H. sapiens codon optimized Integrase Bxb1.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase Bxb1 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Tags

8xHis and EGFP

Expression

Mammalian

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

Plasmid

#85717

Purpose

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Sp dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO and U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHTN-LC3B

Plasmid

#219440

Purpose

Expresses HaloTag-LC3B, an autophagosome marker

Depositor

Erika Holzbaur

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

LC3B (Map1lc3b Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

Plasmid

#85716

Purpose

Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Nme dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO and U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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dmBACCS2-IRES-dOrai-IRES-mCherry

Plasmid

#72894

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-3xFLAG-hTCAB1-puroR

Plasmid

#167459

Purpose

3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

TCAB1 (WRAP53 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Orai1-jGCaMP8f

Plasmid

#231630

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f (ORAI1 Synthetic, Human)

Tags

jGCaMP8f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT2

Plasmid

#127512

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 2.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 2 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT9

Plasmid

#127516

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 9.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 9 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINT13

Plasmid

#127517

Purpose

Plasmid encodes H. sapiens codon optimized Integrase 13.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase 13 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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416 pSG5L HA RB del22

Plasmid

#10721

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del22 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

Deletion of exon 22 (aa738-775)

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pMAL-c5e-Rubicon RH

Plasmid

#216706

Purpose

Bacterial protein expression of Rubicon RH domain

Depositor

James Hurley

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

Rubicon (RUBCN Human)

Tags

MBP

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-695)/pcDNA3.1

Plasmid

#111453

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Kv7.4 (1-695)

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-SOX10

Plasmid

#134669

Purpose

Retroviral expression of human SOX10

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

SRY-box transcription factor 10 (SOX10 Human)

Use

Retroviral

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.5’VIM-eGFP-KI

Plasmid

#170547

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for VIM knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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409 pSG5L HA RB (379-928)

Plasmid

#10734

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (379-928) (RB1 Human)

Expression

Mammalian

Mutation

amino acids 379-928

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
Orai1-jGCaMP8f V203Y

Plasmid

#231631

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f V203Y (ORAI1 Synthetic, Human)

Tags

jGCaMP8f V203Y

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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