We narrowed to 24,010 results for: nes

1. GeNL(Ca2+)_60/pcDNA3

   Plasmid

   #85207

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_60
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Green_CaMBI

   Plasmid

   #124096

   Purpose

   Green calcium-modulated bioluminescent indicator

   Depositor

   Michael Lin

   Article

   Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

   Insert

   Green CaMBI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330S-6

   Plasmid

   #58782

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIM24 KanR

   Plasmid

   #67583

   Purpose

   Plant Gene Expression Vector

   Depositor

   Indu Maiti

   Article

   Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   M24

   Available since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. YeNL_pRSETb

   Plasmid

   #89520

   Purpose

   Yellow color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   YeNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-BRIC

   Plasmid

   #172341

   Purpose

   AAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)

   Depositor

   Huiwang Ai

   Article

   Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

   Insert

   BRIC
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMaCTag-13

   Plasmid

   #119992

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mCherry
   

   Use

   Pcr template

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRFLIP38

   Plasmid

   #65516

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX-AS

   Plasmid

   #188870

   Purpose

   Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants

   Depositor

   Fabio Pasin

   Article

   Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do

   Insert

   none
   

   Use

   Synthetic Biology; Binary t-dna vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid

    #66708

    Purpose

    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

    Depositor

    Charles Gersbach

    Insert

    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Expression

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)/Puro/TagRFP-LL5b

    Plasmid

    #112828

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński, Joshua Sanes

    Article

    Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

    Insert

    LL5b (Phldb2 Mouse)
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-RFP-p2a-postASAP

    Plasmid

    #178793

    Purpose

    Expresses voltage sensor targeted to dendrites and spines along with RFP

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95, ASAP2f and TagRFP
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-cdc2-AF (718)

    Plasmid

    #39872

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    Cdc2 (CDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AF = T14A, Y15F

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pV1200

    Plasmid

    #111429

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM6-mCherry

    Plasmid

    #66534

    Purpose

    Expresses mCherry under the control of the Concensus T7 Promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. OeNL_pRSETb

    Plasmid

    #89529

    Purpose

    Orange color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330A-1x4

    Plasmid

    #58768

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLIK 3XFLAG-EGFP-NLS hygro

    Plasmid

    #87781

    Purpose

    Inducible expression of EGFP with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPD3

    Plasmid

    #118043

    Purpose

    GoldenBraid2.0 Universal Part Domesticator #3

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge