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Showing: 3081 - 3100 of 5008 results

  2. pYZ172

    Plasmid
    #98409
    Purpose
    Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Article
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete lys9 in genome by recombination
    downstream homologous region to delete lys9 in genome by recombination
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98409-plasmid-map-sequence-id-198900

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  3. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

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  6. pRJPaph-eBFP2

    Plasmid
    #67030
    Purpose
    Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Insert
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67030-plasmid-map-sequence-id-115538

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  10. aCE89

    Plasmid
    #204494
    Purpose
    TREtight promoted WBeta and PhlF promoted PhiC31 without PEST tags
    Depositor
    Ron Weiss
    Article
    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.
    Inserts
    φC31 integrase
    WBeta recombinase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    PhIF and TREtight
    Available since
    Dec. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    204494-plasmid-map-sequence-id-409577

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  11. aCE90

    Plasmid
    #204495
    Purpose
    TREtight promoted WBeta and PhlF promoted PhiC31 with PEST tags
    Depositor
    Ron Weiss
    Article
    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.
    Inserts
    phiC31 recombinase
    WBeta recombinase
    Use
    Synthetic Biology
    Tags
    PEST
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    PhIF and TREtight
    Available since
    Dec. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    204495-plasmid-map-sequence-id-409571

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  13. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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  14. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

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  15. CoV2-N-B.1.1.7

    Plasmid
    #177958
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D3L,R203K,G204R,S235F
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177958-plasmid-map-sequence-id-376958

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  16. pENTR_Hu_PRC1(WT)

    Plasmid
    #136349
    Purpose
    Human PCR1 cDNA in Gateway pENTR
    Depositor
    Guillermo de Cárcer
    Insert
    PRC1 (PRC1 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    136349-plasmid-map-sequence-id-270749

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  17. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177939-plasmid-map-sequence-id-348111

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  18. pJRH-1346 U6-B2M sgRNA Gag-pol v2

    Plasmid
    #201917
    Purpose
    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Inserts
    Gag-pol
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201917-plasmid-map-sequence-id-403313

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  19. Zahra78-79

    Plasmid
    #23960
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    R153Q T156R
    Promoter
    Available since
    Feb. 17, 2010
    Availability
    Academic Institutions and Nonprofits only
    23960-plasmid-map-sequence-id-11264

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  20. pOME0276M

    Plasmid
    #182614
    Purpose
    Reversed sequence of Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. TO_7 Control for pOME0275
    Depositor
    Dirk Dittmer
    Insert
    Reverse compliment HHV8 TO_7 control
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182614-plasmid-map-sequence-id-359450

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Showing: 3081 - 3100 of 5008 results