We narrowed to 6,263 results for: wus

1. MTK3_020

   Plasmid

   #123735

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   ERT2-fkbp-spdCa9(205-1368)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Gal4 PGC1 alpha 1-350

   Plasmid

   #8893

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Gal4 DBD

   Expression

   Mammalian

   Mutation

   amino acids 1-350 of PGC-1 alpha

   Promoter

   Available Since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pC1300_pUB10_pco-nCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197981

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-nCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hiv7CMV-Cremyc-2A-GFP

   Plasmid

   #117148

   Purpose

   Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

   Depositor

   Qiang Lu

   Article

   Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

   Insert

   Cremyc-2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221 NLRP1 S1213A

   Plasmid

   #166824

   Purpose

   Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   S1213A

   Promoter

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ239A (dFnCas12a)-TV

   Plasmid

   #136379

   Purpose

   CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   dFnCas12a-TV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D917A

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PDLIM7_PDZ_1

   Plasmid

   #103940

   Purpose

   Protein expression and purification of ENIGMA PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDLIM7 (PDLIM7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. UB061 (FCK-stGtACR2-fusionRed)

   Plasmid

   #135765

   Purpose

   expresses soma targeted GtACR2

   Depositor

   Adam Cohen

   Article

   Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

   Insert

   stGtACR2
   

   Use

   Lentiviral

   Tags

   Fusion Red

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-B4

   Plasmid

   #31708

   Depositor

   Duanqing Pei

   Article

   Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

   Insert

   miR-92a
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-PGC1(1-180)

    Plasmid

    #1028

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains only aa1-180 (the EcoRI site)

    Promoter

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pMXs-MC

    Plasmid

    #31704

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-302b-367
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-dCpf1-BE0

    Plasmid

    #107678

    Purpose

    Expresses dLbCpf1-BE0 in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925/D1148A

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK0_043

    Plasmid

    #123943

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - A1 Insulator - HygroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

    Plasmid

    #197965

    Purpose

    T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-nCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 and pUBQ10

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5 Flag Jnk1a1(APY)

    Plasmid

    #15742

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1(TPF) (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Thr-190Ala

    Promoter

    Available Since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pLenti_dNmeCas9

    Plasmid

    #120077

    Purpose

    dNmeCas9 with no sgRNA cassette lenti construct

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    dNmeCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK3_001

    Plasmid

    #123952

    Purpose

    Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    3XNLS::CRY2PHR::p65
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs-MB

    Plasmid

    #31703

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-106a-363
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV5 Flag Jnk1a1(TPF)

    Plasmid

    #15741

    Depositor

    Roger Davis

    Article

    Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

    Insert

    Jnk1a1(APY) (MAPK8 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Tyr-192Phe

    Promoter

    Available Since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-MtPylRS (human-opti)

    Plasmid

    #164080

    Purpose

    To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

    Depositor

    Tao Liu

    Article

    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

    Insert

    MtPylRS-478(human-opti)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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