We narrowed to 9,586 results for: Coli
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Plasmid#78460PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2264-2315; Q2513R (see deposi…Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1-C
Plasmid#128311PurposeExpress hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tagDepositorInsertHuman nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
TagsHisExpressionBacterialPromoterT7Available SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS3
Plasmid#140626PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP4
Plasmid#129502PurposeExpresses AausFP4 constitutively in E. coli (most strains)DepositorInsertAausFP4
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LplA(AAG)
Plasmid#61820PurposeContains GFP tag for direct live-cell fluorescence imaging of the resofurin ligaseDepositorInsertE. coli lipoic acid ligase
TagsGFPExpressionMammalianMutationE20A, F147A, H149GPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6A (OTU, aa 128-288)
Plasmid#61417PurposeExpresses human OTUD6A (OTU domain) in E. coli.DepositorInsertOTUD6A (OTUD6A Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-127.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM
Plasmid#107163PurposeExpresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzymeDepositorInsertF37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
TagsGSTExpressionBacterialPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
ExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (OTU, aa 133-293)
Plasmid#61419PurposeExpresses human OTUD6B (OTU domain) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-132.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAR69_STRI_m(KS_MAT)_H8_pET22b
Plasmid#122849PurposeExpresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertm(KS_MAT) (Fasn Mouse)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS2
Plasmid#140625PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD2 (OTU, aa 132-314)
Plasmid#61410PurposeExpresses human OTUD2 (OTU domain) in E. coli.DepositorInsertOTUD2 (YOD1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-131 and aa 315-348.PromoterT7Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET29a-hNABP2-N
Plasmid#128308PurposeExpress hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tagDepositorInserthNABP2 N terminal fragment (OB fold) (NABP2 Human)
TagsHisExpressionBacterialMutationN-terminal domain (1–138 aa)PromoterT7Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains3
Plasmid#78459PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
TagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2497-2529PromoterT7Available SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2NhNABP1C
Plasmid#128313PurposeExpress chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tagDepositorTagsHisExpressionBacterialMutationchimeric protein hNABP2N+hNABP1C, Y85C (please se…PromoterT7Available SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRD404
Plasmid#163109PurposeExpression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)DepositorInsertmNeonGreen_H-NSdbd
ExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
His 3C Kinase dead MKK6 1-334
Plasmid#87882PurposeE. coli expression of MKK6 kinase deadDepositorInsertMKK6 (MAP2K6 Human)
Tags3C protease site and 6xHisExpressionBacterialMutationK82A, kinase dead mutantPromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-PD-4xDQNAT
Plasmid#128399PurposePeriplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertH. influenzae protein D
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-69
Plasmid#49815Purposeexpression vector for SRAP(13-69) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-69 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A-Q482A
Plasmid#128946Purposeexpresses R480A Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-OiRS3GG
Plasmid#98589PurposeExpresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).DepositorInsertsgroup I intron
PheS cassette to be selectively replaced by unique asRNA probes
ExpressionBacterialPromoterpBAD and pLtetOAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 G domain
Plasmid#74598Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389DepositorInserthuman dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialPromoterT7Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-87
Plasmid#49816Purposeexpression plasmid for SRAP(13-87) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-87 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.TWIN1.a13-215
Plasmid#49817Purposeexpression vector for SRAP(13-215) in pTWIN1 vectorDepositorInsertsteroid receptor RNA activator protein aa 13-215 (SRA1 Human)
Tagsintein & chitin binding domainExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pD441-H6-SUMO-HyPerFLEX
Plasmid#216676PurposePlasmid with cleavable His-tag for expression of the HyPer-FLEX sensor for hydrogen peroxide in E. coliDepositorInsertHyPerFLEX
Tags6xHis-SUMOExpressionBacterialPromoterT5Available SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His6-MBP-TEV-Rgs14
Plasmid#236395PurposeExpresses His6-MBP-TEV-Rgs14 in E. coliDepositorInsertRattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)
Tagshexahistidine (H6) tag and maltose-binding protei…ExpressionBacterialAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 UBL-12
Plasmid#227230PurposeExpresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 535-775PromoterT7-promoterAvailable SinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7CNW)RS
Plasmid#222874PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIV-Streptomyces
Plasmid#226250PurposeExpresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Streptomyces)
TagsHis6ExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2C
Plasmid#225070PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2C)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2A
Plasmid#225068PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2A)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2B
Plasmid#225069PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2B)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-57
Plasmid#225074PurposeExpresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanII-57)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-6
Plasmid#225076PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanIII-6)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-23
Plasmid#225067PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-23)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-A-V5-His
Plasmid#222849PurposeE. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-A
TagsV5-HisExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
TagsHis10, AP fragment of split APEX2 tagExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-CD28 CAR hinge (I114 – P152)
Plasmid#224929PurposeExpression GST fusion CD28 CAR hinge (I114-P152) in Escherichia coli.DepositorInsertCD28 CAR hinge (I114 – P152) (TMIGD2 Human)
Tagsglutathione-S-transferaseExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-838
Plasmid#217994PurposepLEXSY_I-blecherry3_dom_lacZ Destination VectorDepositorTypeEmpty backboneUseSynthetic BiologyPromoterT7 promotorAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_SAT1 (human)
Plasmid#220477Purposeheterologous expression of SSAT1 under endogenous speG E. coli promoterDepositorAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB-SNAP
Plasmid#166146PurposeGateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10 and SNAPExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB
Plasmid#166147PurposeGateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10-Smt3ExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB-SNAP
Plasmid#166148PurposeGateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10-Smt3 and SNAPExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5(kf)-SNAP
Plasmid#166149PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
TagsHis10-Smt3 and SNAPExpressionBacterialPromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-DDX5(kf)-SNAP
Plasmid#166152PurposeExpression of N. furzeri DDX5 in E. coliDepositorInsertDDX5
TagsHis10 and SNAPExpressionBacterialPromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-HT(C199S)_WPRE
Plasmid#216415PurposeEncodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectorsDepositorInsertoROS-HT(C199S)
UseAAVExpressionMammalianPromoterCAGAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP-eNOSpep-EGFP
Plasmid#205306PurposeExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coliDepositorInsertWHaloCaMP-eNOSpep-EGFP
ExpressionBacterialAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mini v2-HF24_F4
Plasmid#195718PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#4 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceMarch 20, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits