We narrowed to 8,027 results for: Dos;

1. MmSphk1

   Plasmid

   #118599

   Purpose

   Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Mus musculus sphingosine kinase 1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HsSPHK1

   Plasmid

   #118091

   Purpose

   Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens Sphingosine Kinase 1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_dCas9-2xAM

   Plasmid

   #92220

   Purpose

   Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

   Inserts

   Sp-dCas9-2xAM tag
   gRNA to be inserted into Bbs I sites
   

   Use

   CRISPR and Lentiviral

   Tags

   2xAM tag

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   CBh and U6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti6.3-SauriCas9-BlastR

   Plasmid

   #220967

   Purpose

   Expresses human codon-optimized SauriCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SauriCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. STAgR_Neo

   Plasmid

   #102992

   Purpose

   Can be used as backbone for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Type

   Empty backbone

   Use

   CRISPR; Pcr template for stagr vectors

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMMB67EH-Spy

   Plasmid

   #90102

   Purpose

   IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   YcgR (ycgR )
   

   Use

   Broad-host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

   Plasmid

   #173870

   Purpose

   Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-JNK2

   Plasmid

   #86831

   Purpose

   mammalian expression of GFP-JNK2

   Depositor

   Rony Seger

   Article

   Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

   Insert

   JNK2 (MAPK9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-mCherry::LANS4

   Plasmid

   #60785

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS4
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173872

    Purpose

    Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDC158

    Plasmid

    #115793

    Purpose

    Expresses Rat SNAP25

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    SNAP25 (Snap25 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    c84s, c85s, c90s, c92s

    Promoter

    T7

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDC191

    Plasmid

    #115792

    Purpose

    Expresses Rat synaptobrevin 2

    Depositor

    Axel Brunger

    Article

    Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

    Insert

    Synaptobrevin2 (Vamp2 Rat)
    

    Use

    Tags

    hexa histidine

    Expression

    Bacterial

    Mutation

    S28C and C103S (please see comments below)

    Promoter

    T7

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_RBM17_RRM

    Plasmid

    #109786

    Purpose

    Protein expression and purification of RBM17_RRM

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RBM17_RRM (RBM17 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mEmerald-Vinculin-N-21

    Plasmid

    #54304

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pcDNA3.1-mCherry-CyrCR1.miR16

    Plasmid

    #128747

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_ACTG1_Actin

    Plasmid

    #109989

    Purpose

    Protein expression and purification of ACTG1_Actin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ACTG1_Actin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCPP5060

    Plasmid

    #128716

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

    Insert

    shcN-hopN1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-TMCC3

    Plasmid

    #121048

    Purpose

    Expresses human TMCC3 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Insert

    Trans membrane and coiled coil domain containing protein 3 (TMCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid

    #173871

    Purpose

    Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-TMCC2

    Plasmid

    #121047

    Purpose

    Expresses human TMCC2 in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Insert

    Trans membrane and coiled coil domain containing protein 2 (TMCC2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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