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Anti-Synaptotagmin-12 [N277/7R]

Plasmid

#114482

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Bod1l_Sense

Plasmid

#124443

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-DSCP-ftz-FLP(D)

Plasmid

#119894

Purpose

FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Inserts

FLP(D)
DSCP-ftz (ftz Fly)

Use

Drosophila expression

Mutation

a mutated form of FLP, which at position 5 contai…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral N

Plasmid

#113009

Purpose

For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

N terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 1-355

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15A

Plasmid

#113011

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C

Plasmid

#113008

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

amino acids 356-652

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral C 15A

Plasmid

#113007

Purpose

For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

C terminus of tral (tral Fly)

Expression

Bacterial

Mutation

PNG phosphorylation sites mutated to A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Lrrc4c-AP-His

Plasmid

#71959

Purpose

Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4c (Lrrc4c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)R53E/R61E

Plasmid

#60354

Purpose

R53E R61E double mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E and R61E mutations; contains amino acids 7-1…

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188773

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR1-V5/HIS

Plasmid

#205616

Purpose

Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR1 receptor tyrosine kinase (FLT1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KIT-V5/HIS

Plasmid

#201986

Purpose

expression of human KIT receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

KIT receptor tyrosine kinase (KIT Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-Ezrin T567D-BioID2-HA

Plasmid

#227682

Purpose

To over express Phospho-mimetic Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567D

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IGF1R-V5/HIS

Plasmid

#201979

Purpose

expression of human IGF1R receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

IGF1R receptor tyrosine kinase (IGF1R Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RUNX2

Plasmid

#192926

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

RUNX2 (RUNX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#201987

Purpose

expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase (PDGFRA Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hATG5 WT

Plasmid

#111803

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EGFR-V5/HIS

Plasmid

#201102

Purpose

expression of human EGFR receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR5A1

Plasmid

#192915

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR5A1 (NR5A1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-BRCA1

Plasmid

#115491

Purpose

EGFP-BRCA1 expression vector. Parton clone BXY

Depositor

Rob Parton

Article

McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

Insert

BRCA1 (BRCA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S1009R (See depositor comments)

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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