We narrowed to 154,028 results for: ins;
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Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-p21-HA
Plasmid#215110PurposeExpresses p21-HA in mammalian cells from an inducible lentiviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseLentiviralTagsHAExpressionMammalianPromoterCMV (Tet-inducible)Available SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-NLS-HA-TurboID-PCNA
Plasmid#215074PurposeExpresses NLS-HA-TurboID-PCNA in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NLS-HA-TurboID-PCNA
Plasmid#215076PurposeExpresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.DepositorAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PARP1
Plasmid#215136PurposeExpresses mCherry-PARP1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TALEN_HD (SPC right)
Plasmid#126695Purposeright TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start siteDepositorInserthuman SPC right
UseTALENTagsNLS, truncated N term and TAL, FokIExpressionMammalianMutationR451P in SPCPromoterEF1Available SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TALEN_NN (SPC left)
Plasmid#126694Purposeleft TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start siteDepositorInserthuman SPC left
UseTALENTags0.5 repeat NN, TAL, FokI and NLS, truncated N ter…ExpressionMammalianPromoterEF1Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-10xHis
Plasmid#194178PurposeExpresses MPP8-10xHis taggedDepositorAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
Plasmid#154429Purposehu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmidDepositorInserthu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
UseCRISPR and LentiviralExpressionMammalianMutationVRQR point mutations in SpCas9Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p21-HA-NeoR
Plasmid#215104PurposeExpresses p21-HA in mammalian cells.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-FFSS-Cyclin D1
Plasmid#215151PurposeExpresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.DepositorInsertCCND1 (CCND1 Human)
UseLentiviralTagsFFSS (FLAG-FLAG-STREP-STREP)ExpressionMammalianPromoterCMVAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-deltaH-AMBRA1-3xFLAG
Plasmid#174158PurposeVector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)DepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-HsACTB*-thymosinB-8His
Plasmid#111146PurposeExpresses human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integrationTagsThymosin beta and a His-tagExpressionYeastPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-HsACTG1*-thymosinB-8His
Plasmid#111147PurposeExpresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.DepositorInsertactin gamma 1 (ACTG1 Human)
UsePichia pastoris integration vectorTagsThymosin beta and a His-tagExpressionYeastMutationcodon-optimised for expression in Pichia pastorisPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-OGG1
Plasmid#215135PurposeExpresses mCherry-OGG1 in mammalian cells from a retroviral vector.DepositorAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-hCCND1-HA
Plasmid#174154PurposeLentiviral vector expressing HA-tagged human cyclin D1DepositorInsertCCND1 (CCND1 Human)
UseLentiviralTagshemagglutinin (HA)ExpressionMammalianPromoterEF1AAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-AMBRA1-3xFLAG
Plasmid#174157PurposeVector for expression of 3xFLAG-tagged wild-type AMBRA1DepositorAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_RB1#2
Plasmid#174151PurposeLentiviral vector expressing Cas9 and a sgRNA against the human RB1 geneDepositorAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1aL-Cre-IRES-NeoR-W
Plasmid#126700Purposelentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassetteDepositorInsertCre-IRES
UseCre/Lox and LentiviralTagsSV40 NLSPromoterEf1aAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only