We narrowed to 6,263 results for: wus

1. pCMV-MfBulKRS (human-opti)

   Plasmid

   #164196

   Purpose

   To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   Mf BulKRS(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Y269A;Y347F

   Promoter

   CMV

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-1002

   Plasmid

   #31711

   Depositor

   Duanqing Pei

   Article

   Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

   Insert

   miR-302b, c, a, d
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. EF1a_KLF14_P2A_Hygro_Barcode

   Plasmid

   #120500

   Purpose

   Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF14 (KLF14 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTorPE-GEX-GECO1

   Plasmid

   #32464

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEX-GECO1.0
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

   Promoter

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK0_012

   Plasmid

   #123967

   Purpose

   Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hRosa26-BxBI landing pad
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS424-URR

   Plasmid

   #65330

   Purpose

   high copy number plasmid as receiving vector for exogenous pathway

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   URR1-RFP-Leu2-URR2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV-URR2

   Plasmid

   #65332

   Purpose

   standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   URR2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV-URR1

   Plasmid

   #65331

   Purpose

   standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   URR1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-kanMX6-P41nmt1-GST

   Plasmid

   #39287

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

    Plasmid

    #169992

    Purpose

    Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-siriusGFP-FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ141-ZmUbi-RZ-Aa3.8.4

    Plasmid

    #136375

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL421_MLL2_promoter

    Plasmid

    #118696

    Purpose

    Express MLL2/KMT2B promoter for luciferase assay

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    MLL_promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ292 (AaCas12b)-D570A-TV

    Plasmid

    #136380

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pInducer20 CARD8-HA PAM S297A

    Plasmid

    #169983

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, S297A

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEYFP-N1-hRIP3-FL V460P

    Plasmid

    #61379

    Purpose

    Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V460Pmutation (RIPK3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V460 to P

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEYFP-N1-hRIP3-FL V458P

    Plasmid

    #61377

    Purpose

    Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V458Pmutation (RIPK3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V458 to P

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. LV_EF1a_CDK6-P2A-Hygro_Barcode

    Plasmid

    #170224

    Purpose

    Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. JPF0522

    Plasmid

    #124013

    Purpose

    Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_HOXA1_P2A_Hygro_Barcode

    Plasmid

    #120451

    Purpose

    Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HOXA1 (HOXA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. JPF0454

    Plasmid

    #124039

    Purpose

    Encodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pPGK1-spCAS9-P2A-mAzamiGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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