4,181 results

1. pcDNA 3.1(Hygro) myc-hPolQ-Flag

   Plasmid

   #73132

   Purpose

   To express in mammalian cells human PolQ

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   Polymerase theta (POLQ Human)
   

   Tags

   Flag and myc

   Expression

   Mammalian

   Promoter

   cmv promoter

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. psfaMs-Tn5

   Plasmid

   #183637

   Purpose

   Expresses anti-mouse nanobody fused with Tn5

   Depositor

   Goncalo Castelo-Branco

   Article

   Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

   Insert

   anti mouse secondary nanobody fused to Tn5
   

   Tags

   anti-mouse nanobody

   Expression

   Bacterial

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2C::tdTomato Reporter

   Plasmid

   #40281

   Depositor

   Samuel Pfaff

   Article

   Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

   Insert

   2C Promoter (2-cell stage promoter)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Available Since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human Beta-catenin GFP

   Plasmid

   #71367

   Purpose

   mammalian expression of human Beta-catenin GFP

   Depositor

   Alpha Yap

   Article

   Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

   Insert

   Beta-catenin (CTNNB1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-syn-jGCaMP8s-WPRE

   Plasmid

   #162374

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. STAT3-GFP

   Plasmid

   #110495

   Purpose

   reporter of STAT3 signaling activity

   Depositor

   Michael Lewis

   Article

   Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

   Insert

   4 repeats of STAT3 binding site M67 at GFP promoter region
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. T7-VEE-OKS-iM

   Plasmid

   #58972

   Purpose

   RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Inserts

   Oct4 (POU5F1 Human)
   Klf4 (KLF4 Human)
   Sox2 (SOX2 Human)
   cMyc (MYC Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   26S subgenomic promoter and IRES

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-lyso-pHluorin

   Plasmid

   #70113

   Purpose

   Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHluorin (Cd63 Mouse, Aequorea victoria)
   

   Tags

   HA-Tag and pHluorin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLIK-Hygro

    Plasmid

    #25737

    Purpose

    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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