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pAAV-MCS-shCHD5 #1

Plasmid

#68876

Purpose

CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5

Use

AAV and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1 KSR2

Plasmid

#25968

Depositor

Rob Lewis

Article

Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

Insert

Kinase suppressor of Ras 2 (Ksr2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Article

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mIL-2Rα

Plasmid

#59917

Purpose

Expresses mouse interleukin-2 receptor alpha chain

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor alpha (Il2ra Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Bet1

Plasmid

#120243

Purpose

Expresses V5-tagged Bet1 in lentiviral vector

Depositor

Larry Gerace

Insert

Bet1 (Bet1 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-MSulf-1

Plasmid

#13007

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

mouse sulfatase 1 (Sulf1 Mouse)

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
MSCV-HA tagged-Mouse Mdm2 wt-IRES-GFP

Plasmid

#180491

Purpose

Role of Mdm2 splicing variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Insert

mouse Mdm2 full length (wt)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-E29-ChR2GFP2x

Plasmid

#153437

Purpose

AAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E29 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

Chr2-GFP-P2A-GFP

Use

AAV

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCh-CLC

Plasmid

#60035

Purpose

Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2-mCh-Clathrin Light Chain (CRY2 Mouse, Mustard Weed)

Expression

Mammalian

Mutation

M354I, E490G CRY2, A9D*

Promoter

CMV

Available Since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pHalo-MyosinIIA-C25

Plasmid

#128573

Purpose

Studies of cellular contractility

Depositor

Dylan Burnette

Article

Fenix et al Elife. 2018 Dec 12;7. pii: 42144. doi: 1

Insert

Myosin IIA (Myh9 Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Dnmt3l

Plasmid

#13356

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-Stat3

Plasmid

#139805

Purpose

doxycycline-inducible expression of mouse Stat3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Stat3 (Stat3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-PTPR [N165/43R]

Plasmid

#188219

Purpose

Mammalian Expression Plasmid of anti-Pan-PTPR. Derived from hybridoma N165/43

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-PTPR recombinant mouse monoclonal antibody (PTPRF Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE-PtenWT-Blast

Plasmid

#135674

Purpose

Dox-inducible expression (TRE promoter) of wildtype Pten cDNA, Blasticidin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.PI.FLPo.rBG

Plasmid

#236288

Purpose

AAV vector expressing FLPo from TBG promoter

Depositor

Melina Fan, Addgene Research Program

Has Service

AAV8

Insert

FLPo

Use

AAV

Promoter

TBG

Available Since

May 20, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2

Plasmid

#110058

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and mCherry and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only