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Addgene

We narrowed to 8,874 results for: BLI;

Showing: 7801 - 7850 of 8874 results
  1. pT7-7 a-syn A53T D115A mNeonGreen-3K-B11

    Plasmid
    #232017
    Purpose
    Bacterial expression of alpha synuclein A53T mutant, tagged with the final beta strand of mNeonGreen-3K
    Depositor
    Insert
    SNCA (SNCA Human)
    Use
    Tags
    mNeonGreen-3K beta11
    Expression
    Bacterial
    Mutation
    Ala53Thr Asp115Ala
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pCMV mTagBFP2-2A-CHMP2B

    Plasmid
    #232000
    Purpose
    Bicistronic expression of CHMP2B along with an mTagBFP2 transfection marker, connected via 2A sequences.
    Depositor
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pCMV mTagBFP2-2A-CHMP2B-Q165X

    Plasmid
    #232001
    Purpose
    Bicistronic expression of CHMP2B Q165X mutant along with an mTagBFP2 transfection marker, connected via 2A sequences.
    Depositor
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q165X
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pCMV CHMP2B-L4D F5D-3XHA

    Plasmid
    #232003
    Purpose
    Expression of a CHMP2B membrane binding mutant (L4D F5D) with a 3xHA tag.
    Depositor
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    L4D F5D
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. pEF1a FLAG-CHMP2B-d55-96

    Plasmid
    #232013
    Purpose
    Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.
    Depositor
    Insert
    CHMP2B (CHMP2B Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Δ55-96
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pCMV-SNCA-A53T-mClover3

    Plasmid
    #215378
    Purpose
    Expression of the A53T mutant of alpha-synuclein with a C-terminal mClover3 tag.
    Depositor
    Insert
    SNCA (SNCA Human)
    Use
    Tags
    mClover3 tag
    Expression
    Mammalian
    Mutation
    Ala53Thr
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pCMV-SNCA-A53T-mRuby3

    Plasmid
    #215379
    Purpose
    Expression of the A53T mutant of alpha-synuclein with a C-terminal mRuby3 tag.
    Depositor
    Insert
    SNCA (SNCA Human)
    Use
    Tags
    mRuby3 tag
    Expression
    Mammalian
    Mutation
    Ala53Thr
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pTetOFF SNCA-A53T

    Plasmid
    #215372
    Purpose
    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.
    Depositor
    Insert
    SNCA (SNCA Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Ala53Thr
    Promoter
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Expression
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Promoter
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pA-CBh.TP(N55D)-CNOT7-V5H6.MCh.Puro

    Plasmid
    #209949
    Purpose
    In mammalian cells expresses non-binding TP mutant fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    CCR4-NOT transcription complex subunit 7 (CNOT7 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) with a N55D mutation that i…
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pA-CBh-TP-(inactive)CNOT7-V5H6.MCh.Puro

    Plasmid
    #209936
    Purpose
    In mammalian cells expresses TP fused to an inactive CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    Enzymatically inactive CCR4-NOT transcription complex subunit 7 (CNOT7 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pA-CBh-TP-CNOT7(minusCNOT6interaction)-V5H6.MCh.Puro

    Plasmid
    #209940
    Purpose
    In mammalian cells expresses TP fused to a CNOT7 that does not interact with CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    CCR4-NOT transcription complex subunit 7 (Mutation to inhibit interaction with CNOT6) (CNOT7 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pA-CBh-TP-CNOT7(minusCNOT6NOT1interaction)-V5H6.MCh.Puro

    Plasmid
    #209941
    Purpose
    In mammalian cells expresses TP fused to a CNOT7 that does not interact with CNOT6 or NOT1 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    CCR4-NOT transcription complex subunit 7 (Mutation to inhibit interaction with NOT1 and CNOT6) (CNOT7 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pA-CBh-TP-CNOT7(minusNOT1interaction)-V5H6.MCh.Puro

    Plasmid
    #209942
    Purpose
    In mammalian cells expresses TP fused to a CNOT7 that does not interact with NOT1 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Inserts
    CCR4-NOT transcription complex subunit 7 (Mutation to inhibit interaction with NOT1) (CNOT7 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Use
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and CMV
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pcDNA3.1-flag-IARS2-Δ1-48

    Plasmid
    #232341
    Purpose
    Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Insert
    IARS2 (IARS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-48 (indicated as Mito-signal in UniPro…
    Promoter
    CMV
    Available since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid
    #223721
    Purpose
    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell
    Depositor
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  17. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid
    #214468
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid
    #214471
    Purpose
    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S14D S25D S32D T261D
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. GL4.10 E2E1- Prg4 promoter-luciferase

    Plasmid
    #195035
    Purpose
    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
    Insert
    Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. pGL4.10 E3E2E1-Prg4 promoter-luciferase

    Plasmid
    #195036
    Purpose
    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
    Insert
    Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

    Plasmid
    #195037
    Purpose
    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector
    Insert
    pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. Venus-Bik-del72-136-pEGFP-C3

    Plasmid
    #191411
    Purpose
    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).
    Depositor
    Insert
    Bcl-2-interacting killer (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    with deletion of amino acids 72-136 (del72-136) i…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  23. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid
    #140454
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, tyrosine 616 to methioni…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  24. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid
    #140455
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, leucine 624 to asparagine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  25. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid
    #140456
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, alanine 634 to asparagine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  26. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  27. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid
    #140450
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid
    #140452
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, isoluecine 578 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid
    #140453
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, phenylalanine 588 to val…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK

    Plasmid
    #158616
    Purpose
    B6J Id1-LSL-DTA allele targeting vector, low copy number
    Depositor
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of IRES-loxP-NeoR-loxP-DTA after the st…
    Promoter
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  31. GFP-MIOX only

    Plasmid
    #166683
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    GFP-MIOX
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. pTEF1-UDH

    Plasmid
    #166679
    Purpose
    Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Insert
    UDH
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  33. wtVP1 only

    Plasmid
    #166673
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  34. psiCHECK2 3'UTR KGA

    Plasmid
    #110402
    Purpose
    Luminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporter
    Insert
    GLS glutaminase (GLS Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  35. pX330.puro sgRNA KGA Stop1

    Plasmid
    #110405
    Purpose
    CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  36. pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

    Plasmid
    #110408
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. p1A CbbL-

    Plasmid
    #162693
    Purpose
    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk
    Depositor
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    CbbL K194M (inactive rubisco)
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  38. pET14b-dabAB2 D353A

    Plasmid
    #133016
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    D353A
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 7801 - 7850 of 8874 results