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PB-Antares2

Plasmid

#120868

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-Antares2

Expression

Mammalian

Mutation

Antares2 amplicon

Promoter

CAG Promoter

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH NICD1

Plasmid

#86500

Purpose

Mammalian expression of Myc tagged NICD1

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

Cytoplasmic domain only (AA 1753-2531)

Promoter

EF1a

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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M50 Super 8x TOPFlash

Plasmid

#12456

Purpose

Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.GFP

Plasmid

#169308

Purpose

Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 Raptor GFP

Plasmid

#112744

Purpose

express Raptor-GFP

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Raptor-GFP (RPTOR Human)

Use

Lentiviral

Tags

eGFP

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA

Plasmid

#216229

Purpose

S. aureus USA300 GyrA Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB

Plasmid

#216233

Purpose

S. aureus USA300 GyrB Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Akaluc-N1

Plasmid

#120371

Purpose

Akaluc luciferase mammalian expression in -N1 backbone

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc (human optimized)

Expression

Mammalian

Mutation

Human codon optimized

Promoter

CMV

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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4XCLEAR-luciferase reporter

Plasmid

#66800

Purpose

Reporter for activity of the CLEAR network

Depositor

Albert La Spada

Article

Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

Insert

4XCLEAR

Use

Luciferase

Promoter

Four CLEAR elements (GTCACGTGAC) in tandem derive…

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pDY1440 STITCHR GFP NOLC1 payload

Plasmid

#234829

Purpose

GFP cargo with 50bp NOLC homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

copGFP

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCGT003_nuclear-mKate2

Plasmid

#248085

Purpose

Constitutive expression of NLS-tagged mKate2 from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

mKate2

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-ABE8e-dCas9-BSD

Plasmid

#226116

Purpose

Using promoter ef1a expresses ABE8e-dCas9 protein with Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR-SNAP-tag2-KDEL

Plasmid

#226517

Purpose

Mamalian cell expression of SNAP-tag2 in endoplasmic reticulum

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

CalR-SNAP-tag2-KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCGT002_nuclear-EGFP

Plasmid

#248084

Purpose

Constitutive expression of NLS-tagged EGFP from EF1-alpha promoter (for lentiviral packaging)

Depositor

Arjun Raj

Article

Van Eyndhoven et al bioRxiv [Preprint]. 2025 Jun 27:2025.06.

Insert

EGFP

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.Luciferase.IRES.dTomato

Plasmid

#169307

Purpose

Constitutive overexpression of Luciferase cDNA with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-dTomato

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pINEAPLE

Plasmid

#250295

Purpose

Big-IN Payload assembly vector by Golden Gate Cloning using Esp3I. Contains a bacterial green/white GFP-based screening system and a mammalian transient (backbone) BSD selection cassette.

Depositor

Matthew Maurano

Article

Ordoñez et al bioRxiv 2025.08.13.669251

Insert

SuperfolderGFP

Use

Synthetic Biology; Assembly vector for big-in pay…

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET22b_EcSSB_1

Plasmid

#78203

Purpose

Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

Depositor

Martin Webb

Article

Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

Insert

ssb (G26C)

Expression

Bacterial

Mutation

G26C (please see depositor comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_H2B_HaloTag7

Plasmid

#169329

Purpose

Expression of H2B-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag7 (H2BC21 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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