Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 10,975 results for: NSI;

Showing: 6281 - 6320 of 10975 results

  4. pEndo-I-OnuI-TSM

    Plasmid
    #207954
    Purpose
    Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Insert
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in fu…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207954-plasmid-map-sequence-id-412663

    Enlarge

  5. pECNUS2

    Plasmid
    #184885
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    184885-plasmid-map-sequence-id-435252

    Enlarge

  8. pECNUS3

    Plasmid
    #184886
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184886-plasmid-map-sequence-id-437894

    Enlarge

  9. pECNUS1

    Plasmid
    #184884
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184884-plasmid-map-sequence-id-437893

    Enlarge

  13. pGWB602-OEP7-mVenus

    Plasmid
    #213864
    Purpose
    Binary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.
    Depositor
    Yutaka Kodama
    Article
    Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do
    Insert
    chloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213864-plasmid-map-sequence-id-425332

    Enlarge

  14. pCF702

    Plasmid
    #209650
    Purpose
    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus
    Depositor
    Bastian Molitor
    Article
    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7
    Inserts
    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    209650-plasmid-map-sequence-id-416212

    Enlarge

  19. pGG-mTP-TurboID-GFP

    Plasmid
    #209404
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    mTP-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209404-plasmid-map-sequence-id-418443

    Enlarge

  36. mEos3.2trLATo

    Plasmid
    #203746
    Purpose
    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATo (Lat Mouse)
    Use
    Tags
    ER translocation signal - mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203746-plasmid-map-sequence-id-403345

    Enlarge

  38. pLVX-CALPAIN-7-mCherry, F98D

    Plasmid
    #180653
    Purpose
    Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CALPAIN-7
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    siRNA resistant to: GCACCCAUACCUUUACAUU
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180653-plasmid-map-sequence-id-358656

    Enlarge

Showing: 6281 - 6320 of 10975 results