We narrowed to 10,548 results for: CHL

1. pA5c-tesA-vhb50-8fadR

   Plasmid

   #86601

   Purpose

   expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

   Depositor

   Yinjie Tang

   Article

   Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

   Inserts

   tesA
   Vhb50
   fadR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacUV5 and pBAD

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSR0

   Plasmid

   #161984

   Purpose

   Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

   Depositor

   Olga Soutourina

   Article

   Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti CMV/TO Hygro DEST (693-2)

   Plasmid

   #17291

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti CMV/TO Zeo DEST (644-1)

   Plasmid

   #17294

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 2HA-pDEST-C

   Plasmid

   #118374

   Purpose

   Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Hsp68-LacZ-Gateway

   Plasmid

   #37843

   Depositor

   Nadav Ahituv

   Article

   Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

   Type

   Empty backbone

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Mouse Hsp68 minimal promoter

   Available since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti CMV/TO DEST (775-1)

   Plasmid

   #17432

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC-PHYTipi

   Plasmid

   #53283

   Purpose

   Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   crtE, idi, crtY, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   endogenous promoters

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE332

   Plasmid

   #153241

   Purpose

   M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE333

    Plasmid

    #153242

    Purpose

    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJAT17

    Plasmid

    #204298

    Purpose

    For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

    Plasmid

    #187621

    Purpose

    Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    PmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE335

    Plasmid

    #153244

    Purpose

    M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRV2-GG

    Plasmid

    #105349

    Purpose

    virus-induced gene silencing

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    TRV-RNA2(5')
    

    Use

    In planta gene silencing; viral

    Tags

    Expression

    Mutation

    BsaI restriction sites removed

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E1b-GFP-Tol2-Gateway

    Plasmid

    #37846

    Depositor

    Nadav Ahituv

    Article

    Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

    Type

    Empty backbone

    Use

    Zebrafish enhancer assay vector

    Tags

    Expression

    Mutation

    Promoter

    E1b

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLIC-SM-Myc

    Plasmid

    #111189

    Purpose

    High affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)

    Depositor

    Silvia Moreno

    Article

    Hortua Triana et al J Eukaryot Microbiol. 2018 Apr 19. doi:

    Type

    Empty backbone

    Use

    Toxoplasma gondii expression

    Tags

    Spaghetti Monster Myc Tag

    Expression

    Mutation

    Promoter

    Endogenous

    Available since

    Aug. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pUAP4

    Plasmid

    #136079

    Purpose

    Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLenti X2 Blast DEST (w46-2)

    Plasmid

    #17391

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAC-tag-C

    Plasmid

    #108077

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDe-CAS9

    Plasmid

    #61433

    Purpose

    Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PcUBI4-2

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 2HA-pDEST-N

    Plasmid

    #118373

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pScI_dCas9-CDA-UL

    Plasmid

    #108551

    Purpose

    Bacterial Target-AID UGI-LVA vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1-UGI-LVA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PB-TAG-ERP2

    Plasmid

    #80479

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMpGWB300

    Plasmid

    #68628

    Purpose

    MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDGE655

    Plasmid

    #153253

    Purpose

    M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 3TERM_Nos-35S

    Plasmid

    #136116

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_Nos-35S (A. tumefaciens, CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pCBC-MT2T3

    Plasmid

    #50594

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AzoRS-4

    Plasmid

    #182884

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

    Insert

    AzoRS-4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMpGWB104

    Plasmid

    #68558

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMpGWB203

    Plasmid

    #68594

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMpGWB129

    Plasmid

    #68583

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMpGWB201

    Plasmid

    #68592

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJAT11

    Plasmid

    #204290

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti CMV/TO Neo DEST (685-3)

    Plasmid

    #17292

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PROM5_MpEF1a

    Plasmid

    #136113

    Purpose

    Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM5_MpEF1a Mapoly0024s0116
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRU324

    Plasmid

    #167691

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDSW1728

    Plasmid

    #120812

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAF259

    Plasmid

    #105494

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOpt

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    Bitlucopt
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMpGWB204

    Plasmid

    #68595

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    p-T5 Lac

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. 3xFLAG-dCas9/p-bacteria

    Plasmid

    #64325

    Purpose

    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR; Tetracycline-inducible

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    pLtetO-1

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. mNG-sfTq2ox

    Plasmid

    #124219

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2ox
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pGAN230

    Plasmid

    #131122

    Purpose

    reverse yTRAP, RNQ1-aTF reporting on tetR-mKate2

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. MTK2_007

    Plasmid

    #123702

    Purpose

    Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pEF1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pBIG2ab

    Plasmid

    #80616

    Purpose

    pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAC-ZEAX

    Plasmid

    #53274

    Purpose

    Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

    Insert

    crtE, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Deleted idi (ORF6) and crtX genes

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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