We narrowed to 21,630 results for: ESC

1. pcDNA3.1-HA-CDK4

   Plasmid

   #172612

   Purpose

   Expresses HA-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF11-wNIR-GECO2-T2A-HO1

   Plasmid

   #159606

   Purpose

   Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

   Depositor

   Robert Campbell

   Article

   Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

   Insert

   wNIR-GECO2-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   tag-168

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HA-CCND1

   Plasmid

   #172649

   Purpose

   Expresses HA-tagged cyclin D1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET.GltI253-cpGFP.L1LV/L2NP

   Plasmid

   #41733

   Purpose

   a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   T7

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

   Plasmid

   #159676

   Purpose

   Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC364 - pAAV CaMKIIa iRFP

   Plasmid

   #47903

   Purpose

   An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-UBC-S2-cAMPr

   Plasmid

   #160723

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S2-cAMPr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Beatrix

   Plasmid

   #167528

   Purpose

   Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

   Depositor

   Gian Michele Ratto

   Article

   Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

   Insert

   Beatrix
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Chicken Mermaid S188

   Plasmid

   #53617

   Purpose

   Genetically encoded voltage sensor based on mUKG-mKOk FRET pair

   Depositor

   Vincent Pieribone

   Article

   Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

   Insert

   Chicken Mermaid S188
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Gg-VSP contains an R153Q mutation and an amino ac…

   Promoter

   CMV

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOTTC588 - pAAV c-fos Nuc-iRFP

    Plasmid

    #59132

    Purpose

    An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Nuclear-localized Infrared Fluorescent Protein
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    mFos

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-UBC-S3-ExRaiAKAR

    Plasmid

    #160724

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-ExRaiAKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-UBC-S4-ExRaiCKAR

    Plasmid

    #160725

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S4-ExRaiCKAR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-UBC-S2-RAB_EKARev

    Plasmid

    #160726

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S2-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-CCND1

    Plasmid

    #172632

    Purpose

    Expresses untagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL

    Plasmid

    #119952

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-UBC-S3-RAB_EKARev

    Plasmid

    #160727

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S3-RAB_EKARev
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV9-hSyn iCyt_BrEt_SnFR_PM

    Plasmid

    #177757

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn iCyt_BrEt_SnFR_PM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-UBC-S1-jRCaMP1a

    Plasmid

    #160728

    Purpose

    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

    Depositor

    Edward Boyden

    Article

    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

    Insert

    S1-jRCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid

    #159677

    Purpose

    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge