We narrowed to 14,358 results for: cas9 genes

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pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

Plasmid

#211687

Purpose

U6-sgRNA-EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

Plasmid

#197499

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

Plasmid

#185918

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

Plasmid

#223114

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

Plasmid

#109320

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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Cre-responsive Cas9 expressing transposon library vector

Plasmid

#164138

Purpose

Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Synthetic Biology

Expression

Mammalian

Promoter

human U6, EF-1a

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

Plasmid

#113694

Purpose

U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

Plasmid

#120555

Purpose

Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

Cas9, NES, ERT2 and GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

Plasmid

#228252

Purpose

pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

SpCas9 P23H sgRNA, mTagBFP2

Expression

Mammalian

Mutation

n/a

Promoter

CMV and U6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

Plasmid

#140000

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized CBE4max xCas9(3.7) with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

Promoter

CAG

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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1518_pAAV-U6-SA-eGFP-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109316

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against eGFP

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Insert

eGFP gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101104

Purpose

Encodes dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT

Plasmid

#132667

Purpose

Expresses hypoxia-inducible Cas9 and TdT in mammalian cells.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-ODD-T2A-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Promoter

4xHRE_YB TATA

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

Plasmid

#165445

Purpose

Expresses ACX, rPB(8kD) in mammalian cells

Depositor

Wei Leong Chew

Article

Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

Insert

APOBEC-nCas9-rPB(8kD)

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

Plasmid

#210710

Purpose

This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/N580A

Promoter

M11

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

Plasmid

#223115

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

Plasmid

#223116

Purpose

pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP

Use

CRISPR

Tags

BPNLS-P2A-EGFP and BPNLS-TadA8e

Expression

Mammalian

Mutation

TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

Promoter

CMV and T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

Plasmid

#223065

Purpose

Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1

Use

CRISPR

Tags

NLS(SV40)-3xFLAG

Expression

Bacterial

Mutation

Three regions of SpCas9 encode type IIS restricti…

Promoter

Dual T7

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

Plasmid

#204722

Purpose

Constitutive CRISPRi by Zim3-dCas9

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Zim3-dCas9

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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