We narrowed to 7,455 results for: trac
-
-
pLenti-PGK-Venus-Fluc (puro)
Plasmid#140328PurposeLentivirus for expression of Venus-Fluc (firefly luciferase) fusion proteinDepositorInsertVenus-Firefly Luciferase (luc2)
UseLentiviralPromoterhuman PGK promoterAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABEmax-Puro V3
Plasmid#226956PurposeABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLIK CA ROCK2
Plasmid#84649PurposeLentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of VenusDepositorInsertROCK2 (ROCK2 Bovine)
UseLentiviralExpressionMammalianMutationConstitutive Active (please see depositor comment…Available SinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
ch-TOGKDP-GFP
Plasmid#69112PurposeFor mammalian expression of knockdown-proof human ch-TOG tagged with EGFP.DepositorInsertColonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
TagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/TO-hEPO
Plasmid#50437Purposeexpress human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery systemDepositorInsertEPO (EPO Human)
UseLentiviral; Tetracycline-regulatable (t-rex)ExpressionMammalianPromoterCMV/TOAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Beta3
Plasmid#140988PurposeEncodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8s
Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianPromoterhSynAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVSV-G
Plasmid#138479PurposeExpresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.DepositorInsertVSV-G envelop protein
ExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphasS-RLuc8
Plasmid#140980PurposeEncodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasS-RLuc8
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-HsPcdh21-Myc-Flag-His
Plasmid#210818PurposeMammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)DepositorAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma8-GFP2
Plasmid#140990PurposeEncodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 mCherry-hCortKD-Flcortmut3YD
Plasmid#187266PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YF mCherry
Plasmid#187265PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryMutation3YF (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma1-GFP2
Plasmid#140989PurposeEncodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 MS2 sgRNA
Plasmid#92394PurposeChick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCPDepositorInsertchick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
UseCRISPRExpressionMammalianPromoterchick U6.3Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP
Plasmid#115783PurposeThis plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.DepositorInsertmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
UseLentiviralTagscfos basal promoter conjugated MCS5 promoter fuse…PromoterSOX10 Multiple Species Conserved enhancer element…Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pBItetDT240GFP
Plasmid#96904Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalExpressionMammalianPromotertetracycline responsive and bidirectionalAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only