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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,127 results for: trac

Showing: 3141 - 3160 of 7127 results

  3. pcDNA3.1-GGamma11-GFP2

    Plasmid
    #166780
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma11-GFP2 (GNG11 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    166780-plasmid-map-sequence-id-331008

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  8. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

    Plasmid
    #174637
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174637-plasmid-map-sequence-id-346992

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  9. pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)

    Plasmid
    #174636
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174636-plasmid-map-sequence-id-346990

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  13. LV-Sox10MCS5-GFP-EF1a-mCherry

    Plasmid
    #115782
    Purpose
    Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.
    Depositor
    Fraser Sim
    Article
    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1
    Inserts
    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    EF1alpha promoter driven constitutive mCherry
    Use
    Lentiviral
    Tags
    EF1α promoter fused to mCherry and cfos promoter …
    Expression
    Mutation
    Promoter
    SOX10 Multiple Species Conserved enhancer element…
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    115782-plasmid-map-sequence-id-226932

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  15. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Article
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Use
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  17. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chick U6.3
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92394-plasmid-map-sequence-id-191866

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  19. pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

    Plasmid
    #145027
    Purpose
    Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker
    Depositor
    Dorus Gadella
    Article
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Insert
    3xnls-Tq-Ca-FLITS
    Use
    Lentiviral
    Tags
    3xnls-Tq-Ca-FLITS and H2B-Maroon
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    145027-plasmid-map-sequence-id-318435

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  20. AAV-hSyn-Ribo-jGCaMP8s

    Plasmid
    #167572
    Purpose
    DEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Has Service
    AAV1
    Insert
    Ribo-GCaMP8s
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167572-plasmid-map-sequence-id-334463

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Showing: 3141 - 3160 of 7127 results