173,219 results

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pQE30-hPolB

Plasmid

#70761

Purpose

bacterial expression of human DNA Polymerase beta (POLB)

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

POLB (POLB Human)

Tags

6x His

Expression

Bacterial

Mutation

R333L

Promoter

T5/lac operon

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-LC3B WT

Plasmid

#123114

Purpose

Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC9A1_STOP

Plasmid

#161376

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A1 (SLC9A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

Plasmid

#209080

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mBFP-APPP1-mGFP

Plasmid

#196695

Purpose

As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

mEGFP and mtagBFP2

Expression

Mammalian

Mutation

Lys to Val substitution at position 612

Promoter

CMV

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-CreER

Plasmid

#129093

Purpose

KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

CreERT2 and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-cPLA2-mEGFP

Plasmid

#184718

Purpose

Exogenous cPLA2WT

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

cPLA2-mEGFP

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD2

Plasmid

#197880

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD2 (BRD2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Nppa-EGFP

Plasmid

#247327

Purpose

Expresses EGFP under the control of the Nppa promoter

Depositor

William Pu, Mason Sweat

Article

Sweat et al Nat Cardiovasc Res. 2023 Oct;2(10):881-8

Insert

EGFP under the control of the Nppa Promoter

Use

AAV

Expression

Mammalian

Promoter

Nppa proximal promoter

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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