We narrowed to 4,074 results for: p2a

1. pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4

   Plasmid

   #161539

   Purpose

   Constitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-VP64-ZF10
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM574 ZF2x6-C 3xFLAG-NLS-DsDed-ZF1 in TUPV2

   Plasmid

   #161541

   Purpose

   Inducible expression of 3xFLAG-NLS-DsDed-ZF1 under the ZF2x6-C promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsDed-ZF1
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   ZF2x6-C

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-synPCB2.2

   Plasmid

   #139479

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V554L

   Promoter

   CAG promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFETCh_ELK1_iso1

   Plasmid

   #135737

   Purpose

   Donor vector for 3' FLAG tag of human ELK1_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ELK1_iso1 Homology arms (ELK1 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFETCh_MAF1_iso1

   Plasmid

   #135732

   Purpose

   Donor vector for 3' FLAG tag of human MAF1_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   MAF1_iso1 Homology arms (MAF1 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. RV-mCD4-HH7-8

   Plasmid

   #124991

   Purpose

   Express the Helicobacter hepaticus specific TCRa/b HH7-8 in T cell hybridoma cells

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH7-8 TCRa P2A TCRb
   

   Use

   retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. RV-mCD4-HH7-11

   Plasmid

   #124993

   Purpose

   Express the Helicobacter hepaticus specific TCRa/b HH7-11 in T cell hybridoma cells

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH7-11 TCRa P2A TCRb
   

   Use

   retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. RV-mCD4-HH7-9

   Plasmid

   #124992

   Purpose

   Express the Helicobacter hepaticus specific TCRa/b HH7-9 in T cell hybridoma cells

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH7-9 TCRa P2A TCRb
   

   Use

   retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. RV-mCD4-HH7-6

   Plasmid

   #124990

   Purpose

   Express the Helicobacter hepaticus specific TCRa/b HH7-6 in T cell hybridoma cells

   Depositor

   Dan Littman

   Article

   Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

   Insert

   HH7-6 TCRa P2A TCRb
   

   Use

   retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. RV-mCD4-HH5-4

    Plasmid

    #124984

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH5-4 TCRa P2A TCRb
    

    Use

    retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSN28

    Plasmid

    #100113

    Purpose

    expresses a chimeric cMYC-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-cMYC fused to a 'weak' VP16 domain (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN27

    Plasmid

    #100112

    Purpose

    expresses a chimeric cMYC-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-cMYC fused to a 'medium' VP16 domain (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSN26

    Plasmid

    #100111

    Purpose

    expresses a chimeric cMYC-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-cMYC fused to a 'strong' VP16 domain (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFETCh_HOMEZ

    Plasmid

    #86289

    Purpose

    Donor vector for 3' FLAG tag of human HOMEZ

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    HOMEZ homology arms (HOMEZ Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFETCh_HBP1

    Plasmid

    #86272

    Purpose

    Donor vector for 3' FLAG tag of human HBP1

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    HBP1 homology arms (HBP1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCH45 (multiAsCas12a-KRAB lenti)

    Plasmid

    #217330

    Purpose

    Lentiviral expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    UCOE-SFFV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCH61 (multiAsCas12a-KRAB piggyBac)

    Plasmid

    #217332

    Purpose

    piggyBac expression of multiAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBT-PE2-PuroTK-pegRNA_GG

    Plasmid

    #173222

    Purpose

    piggyBac transposon containing both PE2 and a pegRNA cloning site.

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    PuroTK
    pegRNA cloning cassette
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, P2A, and hU6

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRm-NLS-dCas9-GFP11x7-NLS

    Plasmid

    #70224

    Purpose

    Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    NLS-dCas9-GFP11x7-NLS
    

    Use

    Tags

    GFP11x7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_gRNA -hMLHdn-Puro

    Plasmid

    #191105

    Purpose

    Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    I31 silent mutation (ATA to ATC) to remove early …

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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