1,878 results

1. pHW450

   Plasmid

   #198831

   Purpose

   Voltage-sensitive fluorescent protein.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1839

   Plasmid

   #154325

   Purpose

   sgRNA Destination Vector for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJF6

   Plasmid

   #139040

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (includes STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCM1.127

   Plasmid

   #21384

   Depositor

   Geraldine Seydoux

   Article

   Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

   Insert

   pie-1 promoter (short, no ATG) in pDONRP4P1R
   

   Use

   Gateway

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

   Plasmid

   #159813

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF11-wemiRFP670-T2A-HO1

   Plasmid

   #197243

   Purpose

   Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wemiRFP670-T2A-HO1
   

   Expression

   Worm

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKS235

   Plasmid

   #191855

   Purpose

   Negative selection marker for MosSCI

   Depositor

   K Subramaniam

   Article

   Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

   Insert

   peel-1 toxic protein for negative selection against extrachromosomal array
   

   Expression

   Worm

   Promoter

   hsp-16.41

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159850

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAP2

   Plasmid

   #171208

   Purpose

   bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease

   Depositor

   Geraldine Seydoux

   Article

   Schmidt et al bioRxiv 2020.10.15.340570

   Insert

   pgl-3 (pgl-3 Nematode)
   

   Tags

   MBP::HIS::TEV cleavage site

   Expression

   Bacterial

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKS2301

    Plasmid

    #37834

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2cpl-1::YFP (cpl-1 Nematode)
    

    Tags

    YFP

    Expression

    Worm

    Promoter

    nhx-2

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJS145

    Plasmid

    #179159

    Purpose

    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

    Depositor

    Iva Greenwald

    Article

    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Insert

    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    

    Expression

    Worm

    Mutation

    Flexon replacing 2nd intron of 2xNLS::GFP

    Promoter

    rps-27p

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. EFF-1::V5

    Plasmid

    #132961

    Purpose

    Expresses EFF-1 A full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    EFF-1::V5
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ChrII_ttTi5605_loxP_myo2_neoR

    Plasmid

    #194056

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ttTi5605
    

    Expression

    Worm

    Promoter

    None

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW2341

    Plasmid

    #186950

    Purpose

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

    Depositor

    Jordan Ward

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHZ081

    Plasmid

    #27506

    Depositor

    Andrew Fire

    Article

    Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

    Insert

    pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)
    

    Expression

    Worm

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJA281

    Plasmid

    #22513

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    Pmex-5::mCherry (w introns w/o stop)
    

    Expression

    Worm

    Available Since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJH3449

    Plasmid

    #179576

    Purpose

    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Tags

    BFP

    Expression

    Worm

    Promoter

    Plgc-55B

    Available Since

    March 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCM1.53

    Plasmid

    #17250

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

    Insert

    GFP-no stop in pDONR201
    

    Use

    Gateway

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Expression

    Worm

    Promoter

    None

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR49

    Plasmid

    #69259

    Purpose

    phlh-8::tagBFP2

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    TagBFP2
    

    Use

    Cre/Lox

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, TagBFP2 contains 2 sing…

    Promoter

    hlh-8

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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