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We narrowed to 21,102 results for: ACE;

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Showing: 451 - 480 of 21102 results

  1. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid
    #50912
    Purpose
    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C and I730C; N-term deletion of aa13-222; C-t…
    Promoter
    CMV
    Available Since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    50912-plasmid-map-sequence-id-76385

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  2. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    EJ7-GFP variant of EGFP
    Expression
    Mammalian
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113617-plasmid-map-sequence-id-218254

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  3. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    4-μHOM variant of EGFP
    Expression
    Mammalian
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

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  14. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid
    #156415
    Purpose
    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12D (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mTagBFP2
    Mutation
    mutant KRAS4A-G12D
    Promoter
    kdrl
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156415-plasmid-map-sequence-id-308711

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  15. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

    Plasmid
    #156416
    Purpose
    kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mScarlet-I
    Mutation
    mutant KRAS4A-G12V
    Promoter
    kdrl
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156416-plasmid-map-sequence-id-308708

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Showing: 451 - 480 of 21102 results