We narrowed to 1,586 results for: Tef

1. JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

   Plasmid

   #156404

   Purpose

   Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12D (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mTagBFP2

   Expression

   Mutation

   mutant KRAS4A-G12D

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Oliver Weichenrieder, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / Lac

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3EF1aH-myr-Akt

   Plasmid

   #179909

   Purpose

   This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

   Depositor

   Xin Chen

   Article

   Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pαH-rS2d-HexaPro

   Plasmid

   #183515

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pαH-S-GSAS-OMICRON.BA.5

   Plasmid

   #213069

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   S-GSAS-Omicron.BA5 (S )
   

   Use

   Tags

   HRV 3C cleavage site (before tags) C terminal on …

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pαH-S-RRAR-OMICRON

   Plasmid

   #180424

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pαH-S-GSAS-OMICRON.BA.2

   Plasmid

   #184829

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pαH-S-RRAR/XBB.1.16

   Plasmid

   #213037

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   s -RRAR.XBB.1.16 (S )
   

   Use

   Tags

   HRV 3C cleavage site (before tags) 8X His tag 2X…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 furin site RRAR …

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pαH-S-RRAR-OMICRON.BA.5

   Plasmid

   #213071

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pαH-S-RRAR-OMICRON.BA.5 (S )
   

   Use

   Tags

   HRV 3C cleavage site (before tags) C terminal on …

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685, furin site inta…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pαH-S-GSAS-B.1.617.2.v1

    Plasmid

    #182575

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pαH-S-GSAS/EG.5.1

    Plasmid

    #218668

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG.5.1 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pαH-S-GSAS/EG.5.1 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    CMV

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pαH-S-GSAS/XBB.1.5

    Plasmid

    #212991

    Purpose

    "Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pαH-S-GSAS/XBB.1.5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pαH-S-GSAS/XBB.1.16

    Plasmid

    #212992

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pαH-S-GSAS/XBB.1.16 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q, E180…

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pαH-S-GSAS/EG.5

    Plasmid

    #212990

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pαH-S-GSAS/EG.5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

    Plasmid

    #209082

    Purpose

    NFIB and SOX9 mediated astrocye induction

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid

    #213031

    Purpose

    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

    Depositor

    Donna Romero, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    

    Use

    Luciferase

    Tags

    Firefly luciferase antisense intron (3' UTR)

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE bidirectional tet-on

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Use

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pαH-S-GSAS-2P-OMICRON

    Plasmid

    #180593

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pαH-S-GSAS-OMICRON

    Plasmid

    #180423

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pαH-S-RRAR/XBB.1.5

    Plasmid

    #212993

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    Spike S-RRAR/XBB.1.5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); furin site intake; (T19I…

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

    Plasmid

    #209078

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    3K mutant: E35K, E46K, E61K

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid

    #209080

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSrTA1662-toolkit

    Plasmid

    #154372

    Purpose

    Expresses SrTA1662 in plants

    Depositor

    Brande Wulff

    Article

    Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

    Insert

    SrTA1662
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Native promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

    Plasmid

    #209081

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T, NAC deletion (aa60-95)

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETTEV-SENP8

    Plasmid

    #196361

    Purpose

    protein expression of the His-tagged full length protein

    Depositor

    Stefan Knapp

    Insert

    SENP8/NEDP1
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNIC28-BIRC7

    Plasmid

    #196363

    Purpose

    protein expression of the His-tagged full length protein

    Depositor

    Stefan Knapp

    Insert

    BIRC7/Livin
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNIC28-BIRC3

    Plasmid

    #196366

    Purpose

    protein expression of the His-tagged full length protein

    Depositor

    Stefan Knapp

    Insert

    BIRC3/cIAP2
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pILFPZ1A9

    Plasmid

    #223835

    Purpose

    Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mTagBFP>mNeonGreen>
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNIC28-SENP2

    Plasmid

    #196355

    Purpose

    protein expression of the His-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP2 catalytic domain
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    contains only D364-L589

    Promoter

    T7

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pILFPQ13BE5

    Plasmid

    #223836

    Purpose

    Expressing a cytosolic cAMP sensor (mScarletI3-ApacH-StayGold2) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Insert

    pTEF1>mScarletI3>ApacH>StayGold2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKM263-USP7

    Plasmid

    #196365

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    USP7 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S207-E560

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGTVL2-SENP6

    Plasmid

    #196359

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP6 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only E652-A1095

    Promoter

    T7

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKM263-USP40

    Plasmid

    #196356

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    USP40 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only R35-Q383

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGTVL2-SENP3

    Plasmid

    #196357

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP3 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S307-T573

    Promoter

    T7

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pNIC28-SENP1

    Plasmid

    #196354

    Purpose

    protein expression of the His-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP1 catalytic domain
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    contains only E419-L644

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGTVL2-SENP5

    Plasmid

    #196358

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    SENP5 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S492-D755

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pKM263-Rpn13

    Plasmid

    #196360

    Purpose

    protein expression of the GST-tagged PRU domain

    Depositor

    Stefan Knapp

    Insert

    Rpn13 PRU domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only T2-L150

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJY4092

    Plasmid

    #113334

    Purpose

    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Insert

    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSpliceExpress

    Plasmid

    #32485

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDESTsplice

    Plasmid

    #32484

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. R777-E349 Hs.TIAM2

    Plasmid

    #70633

    Purpose

    Gateway ORF clone of human TIAM2 [NM_001010927.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    TIAM2 (TIAM2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. R777-E350 Hs.TIAM2-nostop

    Plasmid

    #70634

    Purpose

    Gateway ORF clone of human TIAM2 [NM_001010927.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    TIAM2 (TIAM2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pDS-Red-N1 GABAA beta3

    Plasmid

    #109143

    Purpose

    The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.

    Depositor

    Stefano Vicini

    Insert

    GABAa receptor
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    beta3 subunit of the GABAa receptor

    Promoter

    CMV

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCAS9i_TRP

    Plasmid

    #141253

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge