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We narrowed to 1,196 results for: fret

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Showing: 751 - 800 of 1196 results

  1. mScarlet-I-LSS-SGFP2

    Plasmid
    #112945
    Purpose
    Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
    Depositor
    Dorus Gadella
    Insert
    LSS-GFP2 mScarlet-I Fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112945-plasmid-map-sequence-id-223563

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  2. mCherry-T2A-LSS-SGFP2

    Plasmid
    #112944
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mCherry and LSS-SGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112944-plasmid-map-sequence-id-220241

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  3. 4MT-D3-CPV

    Plasmid
    #58184
    Purpose
    Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. 2MT-D2-CPV

    Plasmid
    #58183
    Purpose
    Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pVC-FUS-WT

    Plasmid
    #175066
    Purpose
    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175066-plasmid-map-sequence-id-372335

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  8. pVC-FUS-R521C

    Plasmid
    #175067
    Purpose
    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    changed Arginin 521 to Cystein; mVenus: C-Termina…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175067-plasmid-map-sequence-id-362347

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  10. pFUS-WT-VC

    Plasmid
    #175068
    Purpose
    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available Since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175068-plasmid-map-sequence-id-353791

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  15. mScarlet-I-T2A-LSS-SGFP2

    Plasmid
    #112946
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mScarlet-I and LSS-SGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112946-plasmid-map-sequence-id-223584

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  16. pcDNA3.1-Clover-mRuby2

    Plasmid
    #49089
    Purpose
    Clover-mRuby2 tandem fusion for FRET calibration
    Depositor
    Kurt Beam
    Insert
    Clover-mRuby2 fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    49089-plasmid-map-sequence-id-114654

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  17. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

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  18. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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  19. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

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  20. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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  21. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  22. pSBbi-Pur-AktAR2-D

    Plasmid
    #125196
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2-D
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125196-plasmid-map-sequence-id-243601

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  24. Venus-79aa-ActA-pEGFP-C1

    Plasmid
    #166763
    Purpose
    To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166763-plasmid-map-sequence-id-328851

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  25. Epac-S-H151

    Plasmid
    #170346
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    SP6
    Available Since
    Aug. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    170346-plasmid-map-sequence-id-336135

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  32. pUC-GW-Kan-VPC

    Plasmid
    #244163
    Purpose
    Reporter for human p38alpha activation state by FRET
    Depositor
    Thomas Kuhlman
    Insert
    MAPK14/p38a (MAPK14 Human)
    Use
    Synthetic Biology
    Tags
    mCerulean3 and mVenus
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    Sept. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    244163-plasmid-map-sequence-id-487534

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  33. Epac-S-H127

    Plasmid
    #170343
    Purpose
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Insert
    6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170343-plasmid-map-sequence-id-344642

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  34. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid
    #125200
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV-D
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125200-plasmid-map-sequence-id-243591

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  40. pcDNA-mito-ZapCY1

    Plasmid
    #58996
    Purpose
    Mitochondrial Zn-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Park et al ACS Chem Biol. 2012 Aug 10.
    Insert
    mito-ZapCY1 high affinity Zn(II) sensor
    Tags
    See comment
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. Venus-7aa-Cb5-pEGFP-C1

    Plasmid
    #166764
    Purpose
    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5a Rat)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166764-plasmid-map-sequence-id-328853

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  46. pcDNA-mito-ZifCV1.49

    Plasmid
    #59009
    Purpose
    Mitochondrial Zn-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Park et al ACS Chem Biol. 2012 Aug 10.
    Insert
    mito-ZifCV1.49 genetically encoded Zn(II) sensor
    Tags
    See comment
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. Venus-7aa-MAO-pEGFP-C3

    Plasmid
    #166765
    Purpose
    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    MAO tail anchor (MAOA Human)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166765-plasmid-map-sequence-id-328859

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  48. pcDNA-mito-ZifCV1.173

    Plasmid
    #59010
    Purpose
    Mitochondrial Zn-binding FRET biosensor
    Depositor
    Amy Palmer
    Article
    Park et al ACS Chem Biol. 2012 Aug 10.
    Insert
    mito-ZifCV1.173 genetically encoded Zn(II) sensor
    Tags
    See comment
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 751 - 800 of 1196 results