We narrowed to 14,014 results for: TIM

1. pET-15b_T. maritima His-tRNase Z

   Plasmid

   #242436

   Purpose

   Expresses T. maritima tRNase Z in E. coli.

   Depositor

   Sebastian Maerkl

   Article

   Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

   Insert

   tRNase Z
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM-60-HsAGO2-448-859-optim_K

   Plasmid

   #146729

   Purpose

   Bacterial Expression of HsAGO2_448-859-optim

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO2_448-859-optim (AGO2 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-ZmIAA29 (yeast codon optimized)

   Plasmid

   #188441

   Purpose

   Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

   Depositor

   Britney Moss

   Article

   Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

   Insert

   Auxin Signaling Repressor IAA29 (yeast codon optimized)
   

   Use

   Cloning

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-732pUC19-tNGFR-P2A-STIM1

   Plasmid

   #186119

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   STIM1 (STIM1 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. px458-AMBRA1 (optimized for N-terminal knock-in)

   Plasmid

   #172608

   Purpose

   Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against AMBRA1 (AMBRA1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONRPEX18Km-SceI-pheS

   Plasmid

   #111610

   Purpose

   Gateway compatible allelic exchange vector for Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Type

   Empty backbone

   Use

   Allelic exchange in burkholderia sp.

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDAI-SceI-pheS

   Plasmid

   #111611

   Purpose

   Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Insert

   pheS
   

   Expression

   Bacterial

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCdrA-gfp(ASV)C

   Plasmid

   #111615

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Expression

    Bacterial

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. miniTn7(Gm)PA1/04/03-eyfp-a

    Plasmid

    #111620

    Purpose

    eYFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-eyfp
    

    Expression

    Bacterial

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Expression

    Bacterial

    Mutation

    A52V

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. miniTn7(Gm)PA1/04/03-ecfp-a

    Plasmid

    #111619

    Purpose

    eCFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-ecfp
    

    Expression

    Bacterial

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC37

    Plasmid

    #111618

    Purpose

    Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

    Depositor

    Tim Tolker-Nielsen

    Article

    Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

    Insert

    PpqsA-gfp(ASV)
    

    Expression

    Bacterial

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONRPEX18Gm-SceI-pheS

    Plasmid

    #111609

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. CycK-GFP (1-270AA) in Cilantro (Codon optimized)

    Plasmid

    #169930

    Purpose

    Overexpression of 1-270AA CycK-GFP fusion

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Insert

    CCNK (CCNK Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

    Plasmid

    #193942

    Purpose

    a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

    Depositor

    Greg Delgoffe

    Article

    Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

    Insert

    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
    

    Use

    Retroviral

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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