We narrowed to 1,094 results for: gibson assembly
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Plasmid#216928PurposeMega' with knockout of GvpG and GvpTDepositorInsertMega' with knockout of GvpG and GvpT
ExpressionBacterialAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaGK
Plasmid#216926PurposeMega' with knockout of GvpG and GvpKDepositorInsertMega' with knockout of GvpG and GvpK
ExpressionBacterialAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaGJ
Plasmid#216927PurposeMega' with knockout of GvpG and GvpJDepositorInsertMega' with knockout of GvpG and GvpJ
ExpressionBacterialAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaTU
Plasmid#216944PurposeMega' with knockout of GvpT and GvpUDepositorInsertMega' with knockout of GvpT and GvpU
ExpressionBacterialAvailable SinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Light chain
Plasmid#216297PurposeExpresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S FabDepositorInsert39S Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Light chain
Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP
Plasmid#185679PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.DepositorInsertERmembrane-2xHaloTag-KKMP
ExpressionMammalianAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)
Plasmid#137110PurposeiFLinkC Linker cloningDepositorInsertMBP-CS(TEV)-StrepTag-II-AI(TVMV)
UseSynthetic BiologyAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-weak
Plasmid#108316PurposeSynthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.DepositorInsertRFP under controlf of weak synthetic constitutive promoter pFAB4024
ExpressionBacterialPromoterpFAB4024Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MZB117
Plasmid#228481Purpose"Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)DepositorTypeEmpty backboneTagsGST (glutathione S-transferase)ExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
P18m
Plasmid#120941PurposeMoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C31m
Plasmid#120966PurposeMoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.DepositorInsertbxb1 integrase
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P9m
Plasmid#120937PurposeMoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertPr-OR1-OR2
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MZB186
Plasmid#228489PurposeEmpty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)-AvitagExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
U9m
Plasmid#121022PurposeMoClo golden gate assembly BC part for BCD22 (very low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD22
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T1m
Plasmid#121028PurposeMoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).DepositorInsertT500_noGap, short attachment (T11)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C113m
Plasmid#121012PurposeMoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.DepositorInsertCas9
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U2m
Plasmid#121015PurposeMoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD11
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U3m
Plasmid#121016PurposeMoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD13
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only