4,235 results

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pET MBP His6 LIC cloning vector (2Cc-T)

Plasmid

#37237

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Vps34-Flag

Plasmid

#24398

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Vps34 (PIK3C3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-cyto-GCaMP6f

Plasmid

#52925

Purpose

Fluorescent reporter for calcium signaling

Depositor

Baljit Khakh

Article

Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

Has Service

AAV5

Insert

GCaMP6f

Use

AAV

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP46

Plasmid

#22584

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP46 (USP46 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Article

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pBI-EGFP-MnSOD

Plasmid

#16612

Depositor

Bert Vogelstein

Article

Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

Insert

MnSOD (SOD2 Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCalN-ddGFP-A

Plasmid

#40290

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

Calnexin (CANX Human)

Tags

ddGFP-A

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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FUtdTW

Plasmid

#22478

Depositor

Connie Cepko

Article

Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

tandem dimer tomato

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42-wt

Plasmid

#12975

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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Dyn2-pmCherryN1

Plasmid

#27689

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Dynamin 2 (DNM2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-KR

Plasmid

#8985

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

K100R, kinase-inactive

Available Since

Jan. 4, 2006

Availability

Academic Institutions and Nonprofits only

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DsRed-rab7 DN

Plasmid

#12662

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

T22N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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cytoplasmic EKAR (Cerulean-Venus)

Plasmid

#18679

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos36)GFP

Plasmid

#62937

Purpose

Expression of 6xHis-(+36)GFP in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(pos36)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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CA-NFAT2

Plasmid

#11102

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT2 (Nfatc1 Mouse)

Use

Retroviral

Tags

GFP and HA

Expression

Mammalian

Mutation

Constitutively active: multiple serine to alanine…

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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