4,178 results

1. pCMV-Xist-PA

   Plasmid

   #26760

   Depositor

   Rudolf Jaenisch

   Article

   Wutz et al Mol Cell. 2000 Apr . 5(4):695-705.

   Insert

   Xist (Xist Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCitrine-N1

   Plasmid

   #54594

   Purpose

   Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

   Depositor

   Michael Davidson, Roger Tsien, Robert Campbell, Oliver Griesbeck

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Type

   Empty backbone

   Tags

   mCitrine

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. IKK-2 WT

   Plasmid

   #11103

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLifeAct_mScarlet-i_N1

   Plasmid

   #85056

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   LifeAct
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. XBP-1 2: pFLAG.XBP1p.CMV2

   Plasmid

   #21833

   Depositor

   David Ron

   Article

   Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

   Insert

   XBP-1 processed/spliced (Xbp1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC1-HyPer-3

   Plasmid

   #42131

   Depositor

   Vsevolod Belousov

   Article

   Bilan et al ACS Chem Biol. 2012 Dec 20.

   Insert

   HYPER 3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. phage-ubc-nls-ha-tdMCP-gfp

   Plasmid

   #40649

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   tdMCP-gfp
   

   Use

   Lentiviral

   Tags

   EGFP, HA, and NLS

   Expression

   Mammalian

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-R-GECO1.2

   Plasmid

   #45494

   Purpose

   Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   R-GECO1.2
   

   Expression

   Mammalian

   Mutation

   R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

   Promoter

   CMV

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLDLR-Luc (aka: pES7)

    Plasmid

    #14940

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO-PTEN-shRNA-3001

    Plasmid

    #25639

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    PTEN-shRNA-3001 (PTEN Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABE puro mouse IRS-1 myc

    Plasmid

    #11374

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-1 (Irs1 Mouse)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin4A (CUL4A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBMN YFP-DHFR(DD)

    Plasmid

    #29326

    Depositor

    Thomas Wandless

    Article

    Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

    Insert

    YFP-DHFR(DD)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Mutation

    Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

    Available Since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. xCas9(3.7)-BE4

    Plasmid

    #108381

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE4
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CLuc-FKBP

    Plasmid

    #31184

    Depositor

    David Piwnica-Worms

    Article

    Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    C-terminus of Firefly Luciferase and FK506-binding protein 12 (FKBP1A Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVITRO1-dV-IgG1/κ

    Plasmid

    #52213

    Purpose

    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

    Plasmid

    #141374

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCMV-mCherry-MHC-IIA

    Plasmid

    #35687

    Depositor

    Venkaiah Betapudi

    Article

    Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

    Insert

    MHC IIA (MYH9 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 3544 pMIG Bcl-2

    Plasmid

    #8793

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge