We narrowed to 10,458 results for: T7

1. pJKW0826

   Plasmid

   #119027

   Purpose

   E.coli expression plasmid for PmKOMT2

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   PmKOMT2
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST50Trc4-STRNDHFR

   Plasmid

   #63996

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Strep II (STR); TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBL7034

   Plasmid

   #133869

   Purpose

   AtzA expression plasmid

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2020 Mar 20;9(3):671-677

   Insert

   AtzA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 5FAI (EMG1)

   Plasmid

   #79519

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EMG1 (EMG1 Human)
   

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pVY-18 TC-sfGFP

   Plasmid

   #194632

   Purpose

   fluorescent protein with tetracysteine tag

   Depositor

   Allie Obermeyer

   Article

   Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

   Insert

   tetracysteine-sfGFP
   

   Use

   Tags

   MGCCPGCCGGA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETb-pHlash

   Plasmid

   #61550

   Purpose

   Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification

   Depositor

   Carl Johnson

   Article

   Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

   Insert

   pHlash
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pST50Trc1-HISDHFR

   Plasmid

   #63935

   Purpose

   Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hec1 mCherry Plk1 K82R

   Plasmid

   #45206

   Depositor

   Michael Lampson

   Article

   Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

   Insert

   Hec1-targeted mCherry Plk1 K82R
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Plk1 K82R mutation

   Promoter

   T7

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21b-RL018A

   Plasmid

   #42137

   Depositor

   Sheref Mansy

   Article

   Lentini et al ACS Synth Biol. 2013 Mar 15.

   Insert

   mCerulean-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28b-Sad1

    Plasmid

    #90297

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TAL2044

    Plasmid

    #39402

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    eGFP-TALEN-23-Left
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TAL2006

    Plasmid

    #39364

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    eGFP-TALEN-04-Left
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pAW-SpyCas1

    Plasmid

    #113248

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas1
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW-SpyCas2

    Plasmid

    #113249

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas2

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas2
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSARSF505

    Plasmid

    #46832

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    GFPN-NpuDnaE_intein-GFP_C
    

    Use

    Tags

    GFP_C and H6-GFP_N

    Expression

    Bacterial

    Mutation

    stemmer GFP with superfolder mutations S30R and Y…

    Promoter

    T7

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRD324

    Plasmid

    #198045

    Purpose

    Expression of HMfB D14A K30A E34A in E. coli

    Depositor

    Remus Dame

    Article

    Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

    Insert

    HMfB D14A K30A E34A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST50Trc3-STRNDHFR

    Plasmid

    #63978

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 234_pSKB3-Venus-LUMP

    Plasmid

    #65975

    Purpose

    Bacterial expression of Venus-LUMP FRET pair

    Depositor

    Gerard Marriott

    Article

    Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

    Insert

    LUMP
    

    Use

    Tags

    Venus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. His6_BcLOV4_Q355N_mCherry_BamUK

    Plasmid

    #119762

    Purpose

    Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

    Depositor

    Brian Chow

    Article

    Glantz et al Methods Enzymol. 2019;622:249-270. doi:

    Insert

    BcLOV4-Q355N_mCherry
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Mutated glutamine at position 355 to asparagine

    Promoter

    T7

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBzCas13b

    Plasmid

    #164862

    Purpose

    Inducible expression of BzCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    Bergeyella zoohelcum Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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