Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 63,680 results for: cloning

Clear keywords
Showing: 3181 - 3200 of 63680 results

  2. Lenti-SAM-Cre

    Plasmid
    #198714
    Purpose
    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells
    Depositor
    Tyler Jacks
    Publication
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    Pgk and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198714-plasmid-map-sequence-id-402628

    Enlarge

  4. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Publication
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

    Enlarge

  5. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    James Haber
    Publication
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100955-plasmid-map-sequence-id-193248

    Enlarge

  9. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Publication
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47912-plasmid-map-sequence-id-68534

    Enlarge

  10. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167547-plasmid-map-sequence-id-332733

    Enlarge

  12. Mucolipin1-pHcRed C1

    Plasmid
    #62959
    Purpose
    full length human Mucolipin-1 cloned into pHcRed1 C1
    Depositor
    Paul Luzio
    Publication
    Pryor et al Traffic. 2006 Oct;7(10):1388-98.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mucolipin-1 (MCOLN1 Human)
    Tags
    HcRed1
    Expression
    Mammalian
    Mutation
    Mucolipin-1 has 1 silent mutation compared to the…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62959-plasmid-map-sequence-id-383949

    Enlarge

  13. pcDNA3.1+-Tornado-nLuc

    Plasmid
    #212611
    Purpose
    For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.
    Depositor
    Samie Jaffrey
    Publication
    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tornado-CVB3-nLuc
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212611-plasmid-map-sequence-id-420917

    Enlarge

  14. pLenti_dCas9-2xAM

    Plasmid
    #92220
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92220-plasmid-map-sequence-id-181084

    Enlarge

  15. pcDNA3.1+-Tornado-split-nLuc

    Plasmid
    #212612
    Purpose
    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.
    Depositor
    Samie Jaffrey
    Publication
    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tornado-CVB3-split-nLuc
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    C373T mutation in CVB3
    Promoter
    CMV
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212612-plasmid-map-sequence-id-420921

    Enlarge

Showing: 3181 - 3200 of 63680 results