We narrowed to 10,632 results for: t7

1. pMB60

   Plasmid

   #47941

   Purpose

   Can be used to generate synthetic guide in vitro from T7 promotor

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   synthetic guide RNA
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a.MJ0135

   Plasmid

   #11412

   Depositor

   Sung-Hou Kim

   Article

   Lai et al Structure. 2000 Aug 15. 8(8):897-904.

   Insert

   RNase HII (MJ_RS00700 M. jannaschii)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. p15A-OptoCre-cat-T172A-P-R

   Plasmid

   #189854

   Purpose

   Cre-inducible activation of cat-T172A for chloramphenicol resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P-loxP-TT-loxP-R-catT172A
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P (T7 phage gene 10)

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAL2084

   Plasmid

   #39452

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-48-Left
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

5. KaiC

   Plasmid

   #41884

   Depositor

   Michael Rust

   Article

   Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

   Insert

   Kai C
   

   Tags

   histadine

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVhyg-3xT7

   Plasmid

   #171028

   Purpose

   Expresses 3xT7-tagged target in mammalian cells

   Depositor

   Kai Ge

   Article

   Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   3x-T7

   Expression

   Mammalian

   Promoter

   5' LTR

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ClfB_N2N3-HIS-ybbr

   Plasmid

   #101717

   Purpose

   ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ClfB_N2N3
   

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDJSVT89

   Plasmid

   #109303

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Tags

   TamA 1-27, 6His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TAL2052

   Plasmid

   #39410

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   eGFP-TALEN-27-Left
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TAL2053

    Plasmid

    #39411

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    eGFP-TALEN-27-Right
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid

    #208636

    Purpose

    for E. coli expression of human eIF5B (ENST00000289371)

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) (EIF5B Human)
    

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-Rluc-BoxB-A114-N40

    Plasmid

    #50562

    Purpose

    Template DNA for in vitro transcription using T7 polymerase

    Depositor

    Yukihide Tomari

    Article

    Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

    Insert

    Luciferase-BoxB-A114-N40
    

    Use

    Cloning vector

    Promoter

    lac

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. CHD1 (CHD1A-c107)

    Plasmid

    #110253

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CHD1 (CHD1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TAL2085

    Plasmid

    #39453

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    eGFP-TALEN-48-Right
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pRSET-B-frSkyran-S

    Plasmid

    #182209

    Purpose

    Bacterial expression of frSkylan-S

    Depositor

    Shiro Suetsugu

    Article

    Osuga et al Mol Biol Cell. 2021 Nov 1;32(21):br7. do

    Insert

    frSkylan-S
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPQD0KN0245

    Plasmid

    #162284

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-EthR

    Plasmid

    #51170

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    EthR
    

    Use

    Synthetic Biology

    Tags

    6x-his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDJSVT90

    Plasmid

    #109304

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST50Trc1-DHFRHIS

    Plasmid

    #63949

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPQDKN0729

    Plasmid

    #162316

    Purpose

    TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-NET-TEVprotease
    

    Use

    Synthetic Biology

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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