We narrowed to 11,716 results for: Vars
-
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
TagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK671.306
Plasmid#192641PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyPromoterBBa_J23119Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GTT-g
Plasmid#81109PurposeGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSc polyA + Shal1
Plasmid#16196DepositorInsertDrosophila melanogaster Shaker cognate l (Shal Fly)
UseXenopus oocyte expressionMutationN/AAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
GTH-r
Plasmid#81106PurposeGene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST-KNarK2
Plasmid#44565Purposefor ectopic hypoxia inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω1
Plasmid#101710PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-TagRFP675-N1
Plasmid#44276DepositorInsertTagRFP675
Tagschicken paxillinExpressionMammalianMutationM41Q/F80W/S143N/L147M/S158N/D159Y/N173SPromoterCMVAvailable SinceOct. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-CAHS1
Plasmid#90030PurposeTo express GFP-tagged CAHS1 in mammalian cells transientlyDepositorInsertCAHS1
TagsAcGFP1ExpressionMammalianPromoterCMVAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQP-2460
Plasmid#138973PurposeLight-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
Available SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4-2T
Plasmid#28250DepositorInsertsingle chain Cse4-Scm3-H4 with 2 Thrombin site
TagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3. An…Available SinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSopB2xHA
Plasmid#112513PurposePsopB driven sopB-2xHA and sigEDepositorInsertsopB-2xHA/sigE operon
Tags2xHAExpressionBacterialMutationSopB C-terminal 2xHAPromoterPsopBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBIP1::eYFP
Plasmid#135231PurposeUPR signaling reporter (upregulates eYFP expression under UPR conditions)DepositorInsertpBiP1
TagseYFPExpressionPlantPromoterBiP1Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-VSVG-16
Plasmid#55517PurposeLocalization: Golgi/ER Transport, Excitation: 462, Emission: 492DepositorInsertVSVG
TagsmTFP1ExpressionMammalianMutationdeltaK VSVG ts045PromoterCMVAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)
Plasmid#159886PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-1(no_atg)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)
Plasmid#159887PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-2 (no_start)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1359 - Intron GG2 - smu-1
Plasmid#159805PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - smu-1
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only