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pET28a-2xMreBN-mCherry-His

Plasmid

#133626

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

Depositor

Petra Schwille

Publication

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

2xMreB-mCherry-His

Tags

His

Expression

Bacterial

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BSP608

Plasmid

#122253

Purpose

expression vector for mNeptune under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mNeptune

Expression

Worm

Promoter

EFT3

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTK398

Plasmid

#114714

Purpose

mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIplac211-iGFP-YPT31

Plasmid

#105263

Purpose

Plasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt31 (YPT31 )

Tags

iGFP

Expression

Yeast

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTAL1

Plasmid

#31031

Depositor

Adam Bogdanove, Daniel Voytas

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

TALE

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMRS001.2-GFP (control sensor)

Plasmid

#27405

Depositor

David Bartel

Publication

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Type

Empty backbone

Tags

GFP

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

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Dynein-2_complex

Plasmid

#132536

Purpose

Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

Depositor

Andrew Carter

Publication

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Inserts

DYNC2H1 (DYNC2H1 Human)
DYNC2LI1 (DYNC2LI1 Human)
WDR34 (WDR34 Human)
WDR60 (WDR60 Human)
DYNLL1 (DYNLL1 Human)
DYNLL2 (DYNLL2 Human)
DYNLRB1 (DYNLRB1 )
DYNLRB2 (DYNLRB2 Human)
DYNLT1 (DYNLT1 Human)
TCTEX1D2 (TCTEX1D2 Human)
DYNLT3 (DYNLT3 Human)
LC8-like (LOC392067 Human)

Tags

8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-Flot2-GFP

Plasmid

#252028

Purpose

Delivery of Flot2 gene into mammalian cells

Depositor

Thierry Soldati

Publication

Perret et al Sci Adv. 2026 Jan 2;12(1):eady0812. doi:

Insert

Flotillin-2 (FLOT2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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px-458-sgRNA-scramble

Plasmid

#206924

Purpose

Plasmid expressing Cas9, GFP and non-targeting guides for using as a control in CRISPR experiments

Depositor

José Cuezva

Publication

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

non-targeting human sgRNA guides

Use

CRISPR

Promoter

U6

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pJL NarX Myc

Plasmid

#225651

Purpose

Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

Depositor

Neha Kamat

Publication

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NarX (narX E. coli)

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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FB007

Plasmid

#119705

Purpose

Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

Depositor

Jose Marcos

Publication

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Promoter gpdA

Use

Synthetic Biology; Domestication of dna parts for…

Promoter

gpdA

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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lvl0 FAD2t

Plasmid

#153377

Purpose

Plant terminator

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

FAD2 terminator

Expression

Plant

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 nluc CDS

Plasmid

#153404

Purpose

Nanoluciferase coding sequence

Depositor

Naomi Nakayama

Publication

Andreou et al bioRxiv, March 31, 2021

Insert

nanoluciferase CDS

Expression

Plant

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pXNgate21-3HA

Plasmid

#105078

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOGG009

Plasmid

#113983

Purpose

Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Gentamicin

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNXgate32-3HA

Plasmid

#105076

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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FFiBlast p5

Plasmid

#68470

Purpose

Inverted and floxed blasticidin resistance

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)(FRT)MCS

Plasmid

#31377

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(loxP)MCS

Use

Cre/Lox; Flp/frt

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET15p65

Plasmid

#27661

Depositor

Kathleen Collins

Publication

Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

Insert

Tetrahymena telomerase holoenzyme p65

Tags

His

Expression

Bacterial

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only