We narrowed to 3,642 results for: ORF1

Showing: 3221 - 3240 of 3642 results

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pLKO.1-Teton-puro-shOCT4

Plasmid

#198761

Purpose

conditional knockdown of OCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shOCT4 (POU5F1 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Nsp13 K289A-EGFP

Plasmid

#165130

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 K289A (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep128

Plasmid

#136872

Purpose

Mammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep128 (CEP128 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep128

Plasmid

#136823

Purpose

Mammalian expression of the centrosomal protein Cep128 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep128 (CEP128 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA Oct4A -158

Plasmid

#50922

Purpose

U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Oct4A -158 sgRNA (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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TFORF3185

Plasmid

#144661

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TOX2 (TOX2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 2

Plasmid

#22210

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCX4-Oct4

Plasmid

#36116

Depositor

Steven Dowdy

Article

Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

Insert

Oct4 (POU5F1 Human)

Use

Retroviral

Promoter

CMV

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA4TO R99A CoV-2 nsp1 3xFLAG

Plasmid

#176061

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

Plasmid

#157691

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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OCT4 GFP puro Donor 3

Plasmid

#22211

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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Nsp14D-mCherry

Plasmid

#165139

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp14D (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp4 deltaC-EGFP

Plasmid

#165126

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 deltaC (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TOX2

Plasmid

#192920

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TOX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TOX2 (TOX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

Plasmid

#157692

Purpose

mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pD649-HAsp-VSTM2L-COMP5AP-AviTag-9xHis

Plasmid

#157146

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSTM2L (VSTM2L Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-M2O

Plasmid

#188034

Purpose

Fusion of human MYC Transactivation Domain (TAD) MYC Box I/II (MBI/II) with human OCT4 protein expressed in pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

MYC TAD MBI/II region fused with OCT4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN1-EGFP

Plasmid

#165127

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN2-EGFP

Plasmid

#165128

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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