We narrowed to 5,639 results for: pcas

1. pCPH2A.J-GS3

   Plasmid

   #204757

   Purpose

   Expression of Cas9 and human H2A.J

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AJ (H2AJ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. psgRNA-GST-EGFP-GPIBY55

   Plasmid

   #213720

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. psgRNA-GST-EGFP-GPIDAF

   Plasmid

   #213719

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. psgRNA-GST-EGFP-GPICEAM7

   Plasmid

   #213721

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   GST

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLV3Cas-LT2

   Plasmid

   #210550

   Purpose

   Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   1bp insertion after AAVS1 target sequence that di…

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE-RA5.5

   Plasmid

   #208316

   Purpose

   Expresses ABE-RA5.5 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA5.5-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hPRL2_1-163

   Plasmid

   #209794

   Purpose

   Bacterial expression of human PRL-2 without farnesylation motif

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

   Insert

   PRL-2 (PTP4A2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 1-163 only

   Promoter

   T7

   Available since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pROF018

   Plasmid

   #155325

   Purpose

   Constitutive expression of KRAB-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-NLS-KRAB-EL222-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pROF050

   Plasmid

   #155327

   Purpose

   Constitutive expression of BRD-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-BRD-NLS-EL222-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HSF2 KO

    Plasmid

    #200208

    Purpose

    gRNA for HSF2 KO

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HSF2 KO (Hsf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TBPL1 KO 1

    Plasmid

    #200210

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 1 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TBPL1 KO 2

    Plasmid

    #200211

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 2 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197958

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCVp and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

    Plasmid

    #197963

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-vCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26 and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTij_sg_mmu_Med6_C_terminal

    Plasmid

    #197869

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pClgn-Tesmin-BioID2-3xFLAG

    Plasmid

    #186816

    Purpose

    Mouse Clgn promoter-driven expression of TESMIN-BioID2-3xFLAG

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Insert

    Tesmin (Tesmin Mouse)
    

    Use

    Tags

    BioID2-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Clgn promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pClgn-Tesmin-TurboID-3xFLAG

    Plasmid

    #186817

    Purpose

    Mouse Clgn promoter-driven expression of TESMIN-TurboID-3xFLAG

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Insert

    Tesmin (Tesmin Mouse)
    

    Use

    Tags

    TurboID-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Clgn promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. UAS-GFP::NMN-Deamidase{dead}

    Plasmid

    #187869

    Purpose

    Gal4/UAS expression of GFP::NMN-Deamidase(dead)

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    NMN-Deamidase{dead}
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. UAS-4x(tRNA::axed{sgRNA})

    Plasmid

    #187883

    Purpose

    Gal4/UAS sgRNA expression targeting axed

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    4 sgRNAs targeting axed
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UAS-4x(tRNA::hiw{sgRNA})

    Plasmid

    #187884

    Purpose

    Gal4/UAS sgRNA expression targeting hiw

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    4 sgRNAs targeting hiw
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. UAS-4x(tRNA::dsarm{sgRNA})

    Plasmid

    #187885

    Purpose

    Gal4/UAS sgRNA expression targeting dsarm

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    4 sgRNAs targeting dSarm
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 1137B=Hsp70Bb-Cas9

    Plasmid

    #153284

    Purpose

    The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Dmel Hsp70Bb

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid

    #181748

    Purpose

    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Type

    Empty backbone

    Use

    Substrate plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBI101.1/ProGOXL6:GUS

    Plasmid

    #175562

    Purpose

    Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    ProGOXL6
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    GOXL6

    Available since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pBI101.1/ProGOXL5:GUS

    Plasmid

    #175561

    Purpose

    Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    ProGOXL5
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    GOXL5

    Available since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pBI101.1/ProGOXL4:GUS

    Plasmid

    #175560

    Purpose

    Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    ProGOXL4
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    GOXL4

    Available since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pBI101.1/ProGOXL3:GUS

    Plasmid

    #175559

    Purpose

    Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    ProGOXL3
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    GOXL3

    Available since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pBI101.1/ProGOXL2:GUS

    Plasmid

    #175558

    Purpose

    Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    ProGOXL2
    

    Use

    Tags

    GUS

    Expression

    Plant

    Mutation

    Promoter

    GOXL2

    Available since

    Jan. 13, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pAT15484_HF-ABE8e

    Plasmid

    #174122

    Purpose

    Expresses ABE8e fused with SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-ABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR207-MUM2sp-PelA

    Plasmid

    #170722

    Purpose

    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)

    Depositor

    George Haughn

    Article

    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

    Insert

    Pectin lyase A
    

    Use

    Gateway donor vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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