We narrowed to 17,271 results for: form
-
Plasmid#68211PurposeProvides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.DepositorInsertbar gene
UseUnspecifiedMutationBsaI and BsmBI sites removedPromoterPNosAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema6a.z-Fc-His
Plasmid#72164PurposeExpresses the extracellular region of the Sema6A, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema4f.a-AP-His
Plasmid#72032PurposeExpresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS
Plasmid#121828PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7) (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA
Plasmid#121806PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETaDepositorInsertHA epitope tag-polyA + EF1a::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KH001: pMVP (L3-L2) V5 epitope tag + WPRE
Plasmid#121762PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertV5 epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2A1
Plasmid#67076PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMX-Flag-WIP 460 deletion
Plasmid#11773DepositorInsertWIP aa1-460 (WIPF1 Human)
UseRetroviralTagsflagExpressionMammalianMutationaa461-503 deletedAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ntn5.z-Fc-His
Plasmid#72109PurposeExpresses the entire Netrin 5, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntn5.y-AP-His
Plasmid#71982PurposeExpresses the entire Netrin 5, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ntn5.z-AP-His
Plasmid#71983PurposeExpresses the entire Netrin 5, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 merlin 308-579
Plasmid#11626DepositorInsertmerlin (NF2 Human)
TagsFlagExpressionMammalianMutationCarboxy-terminal common domain of merlin.Available SinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLacIBD::SDRX (GB0673)
Plasmid#68183PurposeProvides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLacI Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg2 (CG510)
Plasmid#139456PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA
Plasmid#108844PurposeInsert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1,NLSExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse c-myc (exon I)
Plasmid#8446DepositorInsertMouse c-myc (exon I) (Myc Mouse)
ExpressionBacterialAvailable SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BTF3
Plasmid#67059PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dnm2a-DN
Plasmid#109572PurposeMultisite gateway vector for 3' tagging with dominant negative Dynamin2aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 SMAD2
Plasmid#67095PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.5-AP-His
Plasmid#72045PurposeExpresses the extracellular region of the Sema6D, isoform 5 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
KZ701: pMVP (L3-L2) AID + polyA
Plasmid#121791PurposepMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to geneDepositorInsertAID + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Venus-TmiR-G13
Plasmid#25741PurposeLentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.DepositorInsertG alpha 13 miR-shRNA
UseLentiviral and RNAiTagsVenusExpressionMammalianAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
KY801: pMVP (L3-L2) P2A-eGFP + polyA
Plasmid#121764PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.DepositorInsertP2A-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TYK2
Plasmid#67133PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LA102: pMVP (L3-L2) P2A-eYFP + WPRE
Plasmid#121778PurposepMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eYFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB012
Plasmid#119709PurposeComplete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMTDepositorInsertPtrpC:hph:Ttub
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA301: pMVP (L3-L2) P2A-Hygro + WPRE
Plasmid#121787PurposepMVP L3-L2 entry plasmid, contains Hygro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene in lentivirus vectorsDepositorInsertP2A-Hygro + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA802: pMVP (L3-L2) AID + WPRE
Plasmid#121794PurposepMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectorsDepositorInsertAID + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO801: pMVP (L5-L4) Hygro-P2A
Plasmid#121718PurposepMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.DepositorInsertHygro-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107322PurposeExpresses SpCas9 (R1335K) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to SaCas9PromoterCMV IE93Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2XNLS-SpCas9MT3-NLS-3xHA-2XNLS-NmCas9WT-2XNLS
Plasmid#107325PurposeExpresses SpCas9 (R1335K) fused to NmCas9 in mammalian cellsDepositorInsertSpCas9-NmCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to NmCas9PromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2XNLS-SpCas9MT2-NLS-3xHA-2XNLS-NmCas9WT-2XNLS
Plasmid#107326PurposeExpresses SpCas9 (R1333S) fused to NmCas9 in mammalian cellsDepositorInsertSpCas9-NmCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1333S) is fused to NmCas9PromoterCMV IE93Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.4-Fc-His
Plasmid#72170PurposeExpresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
M26I DJ-1 TEV site pET15b
Plasmid#60685PurposeRecombinant protein expression of Park7DepositorInsertM26I DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26I and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_huMcl-1.2
Plasmid#85531PurposeInducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporterDepositorInserthu Mcl-1.2
UseCRISPR and LentiviralExpressionMammalianPromoterH1tAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH743-CEN-RLuc/slowstaCFLuc
Plasmid#38223DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.y-Fc-His
Plasmid#72181PurposeExpresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.y-AP-His
Plasmid#72055PurposeExpresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.a-AP-His
Plasmid#72057PurposeExpresses the extracellular region of the Unc5D, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5a.z-AP-His
Plasmid#72051PurposeExpresses the extracellular region of the Unc5A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5a.a-Fc-His
Plasmid#72176PurposeExpresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.a-Fc-His
Plasmid#72183PurposeExpresses the extracellular region of the Unc5D, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) flag Lunatic Fringe (CT#471)
Plasmid#13980DepositorAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BMPR2
Plasmid#67127PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRVH1-hygro shStx3
Plasmid#40071DepositorInsertStx3 shRNA-1
UseRetroviralPromoterH1Available SinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
M26L DJ-1 TEV site pET15b
Plasmid#60686PurposeRecombinant protein expression of Park7DepositorInsertM26L DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26L and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Neo1.c-AP-His
Plasmid#71965PurposeExpresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.z-Fc-His
Plasmid#72182PurposeExpresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) flag Lunatic Fringe (CT#479)
Plasmid#13979DepositorAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.z-Fc-His
Plasmid#72185PurposeExpresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only