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pLKO_AU1.HA.V5_mCherry-NLS

Plasmid

#178221

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HA.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRC/RSV-rat Agt

Plasmid

#122104

Purpose

Expression of angiotensinogen

Depositor

John Chan

Article

Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

Insert

angiotensinogen (Agt Rat)

Expression

Mammalian

Promoter

RSV promoter

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528D-S529D-sgEIF3D

Plasmid

#222127

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528D, S529D

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-CtermDel-sgEIF3D

Plasmid

#222131

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

161 + 527 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA Set1

Plasmid

#192219

Purpose

It expresses HA-fused mouse Set1 isoform protein in mammalian cells

Depositor

Hisakazu Ogita

Article

Molla et al Commun Biol. 2022 Oct 7;5(1):1071. doi:

Insert

Set isoform 1 (Set Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528N, S529N

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*/PBM*

Plasmid

#195221

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

Dual mutant for HRS>AAA / lacking DTHL

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pACB120

Plasmid

#222213

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA(K10N, S11R, W15C, G76V, G258D)

Tags

6x His

Expression

Bacterial

Mutation

Contains five amino acid mutations: K10N, S11R, W…

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-Y36A_P

Plasmid

#147221

Purpose

Mammalian Expression of HsDCP1a-Y36A

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsDCP1a-Y36A (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNkx2-1#1/Cre

Plasmid

#193221

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Nkx2-1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNkx2-1#1 (Nkx2-1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Blast-FLAG-hDIXDC1 S592A

Plasmid

#61221

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Mutation

Changed Serine 592 to Alanine

Promoter

PGK

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
II-AAAG

Plasmid

#43221

Depositor

Chad Cowan, Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Insert

II-AAAG

Use

Bacterial cloning

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-PalmGRET

Plasmid

#158221

Purpose

BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

PalmGFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEN243-EN1953

Plasmid

#224546

Purpose

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl (Nipbl Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0741

Plasmid

#142216

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

JDP2 (JDP2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-GFP

Plasmid

#122100

Purpose

AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV2

Insert

GFP

Use

AAV

Tags

NA

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available Since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-CTNNB1-HA

Plasmid

#242217

Purpose

Expression of full-length human β-catenin using piggyBac transposition

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

CTNNB1 (CTNNB1 Human)

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Blast

Plasmid

#169221

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Blast

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Brg1 Del2 pET28a

Plasmid

#122116

Purpose

Expression Brg1 amino acids 1-837 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

6XHis and His-T7

Expression

Bacterial

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Brg1 Del3 pET28a

Plasmid

#122117

Purpose

Expression Brg1 amino acids 836-1446 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

His-T7

Expression

Bacterial

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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