We narrowed to 10,000 results for: ADA

1. EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191184

   Purpose

   Expression of RPLP1 sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:RPLP1.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ1359 - Intron GG2 - smu-1

   Plasmid

   #159805

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG2 - smu-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

   Plasmid

   #191175

   Purpose

   Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   CMV-TO:mCherry:s70587:EGFP (frameshifted)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is frameshifted

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Psy074

   Plasmid

   #99504

   Purpose

   Bacterial expression of LIPS M1+TE

   Depositor

   Jay Keasling

   Article

   Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

   Insert

   LIPS M1+TE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ2215 - Intron GG3 - 900 bp

   Plasmid

   #159882

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG3 - 900 bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK-flag-USP19 1-493

   Plasmid

   #78581

   Purpose

   Expression flag-USP19 1-493 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 1-493 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutated 494 residue to stop codon

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1-flag-UTR

   Plasmid

   #183234

   Purpose

   Mammalian expression empty backbone

   Depositor

   Jorge Ferrer

   Article

   Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

   Type

   Empty backbone

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-smURFP-P2A-GFP

   Plasmid

   #197151

   Purpose

   Expresses the protein of smURFP-P2A-GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   smURFP-P2A-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5.1B-lambdaN-HA-DmGW182

   Plasmid

   #22406

   Depositor

   Elisa Izaurralde

   Article

   Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

   Insert

   gawky, variant A (gw Fly)
   

   Use

   Drosophila expression

   Tags

   lambdaN-HA

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. EC.SurA.TYB1.a172-274.wt

    Plasmid

    #15934

    Depositor

    David McKay

    Article

    Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

    Insert

    Peptidyl prolyl isomerase domain 1 of SurA (surA E. coli)
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available Since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUASp-Egl-Trbo

    Plasmid

    #179993

    Purpose

    Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

    Depositor

    Graydon Gonsalvez

    Article

    Baker et al Development. 2021 Nov 15;148(22). pii: 2

    Insert

    Egl-Trbo
    

    Use

    Tags

    TurboID

    Expression

    Insect

    Mutation

    Promoter

    UAS-Gal4

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAID1.2-CMV-NGFP-mAID

    Plasmid

    #140605

    Purpose

    Expression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF-HA-Net1

    Plasmid

    #28264

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1 (Net1 Mouse)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. sspa-GCaMP6s

    Plasmid

    #67559

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191183

    Purpose

    Expression of PPIB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:PPIB.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)

    Plasmid

    #191142

    Purpose

    Expression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s17d9b:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-KG/Net1

    Plasmid

    #28260

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1 (Net1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pAID1.2-CMV-NmScarlet-mAID

    Plasmid

    #140618

    Purpose

    Expression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-SLAP-FLAG

    Plasmid

    #74500

    Purpose

    Overexpression of SLAP in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al PLoS One. 2012;7(12):e53509. doi: 10.137

    Insert

    SLAP (SLA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N18K, G74A, and T149A

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only