We narrowed to 15,769 results for: LEA

1. pEGFP-Aurora A

   Plasmid

   #198402

   Purpose

   Expression of EGFP-tagged Aurora A in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Aurora A
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCP-RecT

   Plasmid

   #183827

   Purpose

   pLenti-EF1A-MCP-EXTEN-RecT-NLS

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   MCP-RecT_NLS
   

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSQT1313

   Plasmid

   #53370

   Purpose

   multiplex gRNA expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   None
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF6.mCherry-TSG101

   Plasmid

   #38318

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   TSG101 (TSG101 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A-1x6

   Plasmid

   #58770

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAMBgfp

   Plasmid

   #206130

   Purpose

   This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

   Depositor

   Anne Osbourn

   Article

   Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

   Insert

   hygromycin resistance gene and the Promoter of ToxA+SGFP
   

   Use

   Binary vector for expression of sgfp in filamento…

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. stathmin-GFP

   Plasmid

   #86782

   Purpose

   Mammalian expression of stathmin-GFP

   Depositor

   Lynne Cassimeris

   Article

   Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

   Insert

   stathmin (STMN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

   Plasmid

   #205277

   Purpose

   Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Tags

   HA, NLS(Makkerh)

   Expression

   Mammalian

   Mutation

   C310R/D487K/T513K

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_SUMO_AcCas12n

   Plasmid

   #203808

   Purpose

   pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   SUMO_AcCas12n
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBzCas13b-gRNA-NT

    Plasmid

    #164861

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-NT
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBzCas13b-gRNA-1

    Plasmid

    #164858

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    BzCas13b-gRNA-1
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-LMO-IRES-EGFP

    Plasmid

    #178309

    Purpose

    Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

    Insert

    Lactate 2-monooxygenase
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Nup88

    Plasmid

    #64283

    Purpose

    GFP-tagged mammalian expression of full-length Nup88

    Depositor

    Birthe Fahrenkrog

    Article

    Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

    Insert

    Nup88 (NUP88 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACS129

    Plasmid

    #204594

    Purpose

    Histone H3.1-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 (H3C3 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Reb_206 aka PAS629

    Plasmid

    #71234

    Purpose

    Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    mNeonGreen to second copy RebB, soluble mCherry a…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT378_pattB-pCA-GFP-pA-attB

    Plasmid

    #52554

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCN-EF2132tet

    Plasmid

    #107191

    Purpose

    Expresses EF2132 recombinase for performing recombineering in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    EF2132
    tetR
    

    Expression

    Bacterial

    Promoter

    p23

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-RPS6 (pDD1902)

    Plasmid

    #52913

    Purpose

    contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

    Depositor

    Dirk Dittmer

    Article

    Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

    Insert

    HAS6 (RPS6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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