We narrowed to 12,214 results for: LEA
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Plasmid#31035DepositorInsertTALEN-FokI nuclease backbone
TagsAcV5 and FokI nucleaseAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR-OMICRON
Plasmid#180424PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intactDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ329: pCMV-MmeFz2
Plasmid#205266PurposeHuman expression vector for human codon optimized MmeFz2DepositorInsertMmeFz2
ExpressionMammalianAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_eGCaMP(2+)
Plasmid#201148Purposeexpresses eGCaMP2+ in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGCaMP2+
ExpressionMammalianAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBirA-P19
Plasmid#127919PurposeTransient expression of biotin ligase BirA and p19 in plant protoplastDepositorPublicationInsertBirA
ExpressionPlantPromoter35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLIGFP_W220F
Plasmid#46772PurposeProtein overexpression with the modified LacIWF repressorDepositorInsertLacI_W220F (lacI )
MutationTryptophan 220 replaced by PhenylalanineAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDZ645 pKAN 1x-mCherry-12xPP7 V4
Plasmid#73173PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert1x-mCherry
ExpressionMammalian and YeastAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046916
Plasmid#145627PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046833
Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJOP-HTT-HR30Q
Plasmid#92247PurposeHTT donor DNA with 30Q for homologous recombination-mediated gene modificationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorMutationBarcode of wobble bases to prevent TALEN cleaving…Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLTR.gp140/EGFP.Revdelta38/DsRed
Plasmid#115775PurposeHIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEnvelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
TagsEGFP; dsReDExpressionMammalianMutationgp140 uncleaved (1190-91aa, KR > TG; truncated…PromoterHIV-1 (LTR)Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(KD)
Plasmid#84640PurposemCherry-tagged, LKB1(K78I); kinase-dead mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-LBK1(K78I) (STK11 Human, Synthetic)
Tags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationLysine 78 mutated to Isoleucine; kinase deadPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorPublicationInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1 S449A/T450A/S454A
Plasmid#19847DepositorInsertMKL1 S449A/T450A/S454A (MRTFA Human)
UseRetroviralTags3xFLAGMutationchanged Serine 449, Threonine 450 and Serine 454 …Available sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Citrine2
Plasmid#110772PurposeYellow fluorescent protein with improved photostabilityDepositorInsertCitrine2
TagsHis tagExpressionBacterialAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4
Plasmid#80601PurposeExpresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4
UseAAVExpressionMammalianPromoterCBAAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZHY500
Plasmid#36186DepositorPublicationInsertFokI homodimeric Nuclease domain
TagsACV5ExpressionYeastMutationdelta152,+63 truncation to TAL backbonePromoterTEVAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
Caspase-6
Plasmid#100102PurposeBacterial expression of Caspase-6DepositorAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only