We narrowed to 18,420 results for: REV

1. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-LbNOX

   Plasmid

   #75285

   Purpose

   This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   LbNOX
   

   Tags

   C-terminal FLAG tag with linker

   Mutation

   Human codon optimized

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLOX-TERT-iresTK

   Plasmid

   #12245

   Purpose

   2nd generation lentiviral vector for reversible immortalization of human primary cells

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   TERT, HSV1-TK (TERT Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   LoxP in 3' LTR.

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. Profiline

   Plasmid

   #89925

   Purpose

   Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

   Depositor

   Antoine Jégou, Guillaume Romet-Lemonne

   Article

   Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

   Insert

   profiline-1 (PFN1 Human)
   

   Tags

   None

   Expression

   Bacterial

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS24

   Plasmid

   #58952

   Purpose

   Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

   Depositor

   Thomas Fox

   Article

   Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

   Insert

   Mitochondrially coded Arg8 protein
   

   Mutation

   Coding sequence synthetic in S. cerevisiae mitoch…

   Promoter

   n/a

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCEC-red

   Plasmid

   #196040

   Purpose

   Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

   Depositor

   Paola Branduardi

   Article

   Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Grna cloning

   Expression

   Yeast

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREDTAI

   Plasmid

   #51627

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP92

    Plasmid

    #83549

    Purpose

    Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TDH3 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFPB5A

    Plasmid

    #83556

    Purpose

    Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    GAL1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZH81 pET21b Rat1-His

    Plasmid

    #231650

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    Rat1 (RAT1 Budding Yeast)
    

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115211

    Purpose

    Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PsmCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFP91

    Plasmid

    #83548

    Purpose

    Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PGK1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZS157 CRISPEY RT/Cas9

    Plasmid

    #114454

    Purpose

    Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Insert

    SpCas9, Ec86-RT
    

    Use

    CRISPR

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    S. cerevisiae codon optimized

    Promoter

    GAL1-GAL10

    Available Since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMKO.1 puro hTERT shRNA

    Plasmid

    #10688

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    hTERT shRNA (TERT Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115209

    Purpose

    Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PbuCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pREDIA

    Plasmid

    #51625

    Purpose

    E. coli genome modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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