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pMOS003-lenti-CMV-GCaMPer

Plasmid

#65227

Purpose

ER calcium sensor for lentivirus production

Depositor

Adam Cohen, Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

GCaMPer

Use

Lentiviral

Tags

circularly permuted GFP

Expression

Mammalian

Mutation

GCaMP3 (D324G, D360G, D397G, D435G)

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-3xFLAG

Plasmid

#69837

Purpose

This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NfiB

Plasmid

#64900

Purpose

Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

NfiB (Nfib Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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ins:CFP-NTR

Plasmid

#66593

Purpose

Specific and conditional cell ablation of beta cells in the zebrafish pancreas

Depositor

Didier Stainier

Article

Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

Insert

Insulin promotor driving CFP-nitroreductase

Use

Zebrafish expression

Tags

CFP fused with N-terminus of nitroreductase

Promoter

Zebrafish Insulin promoter (approximately 1.2kb)

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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BPK2139

Plasmid

#65776

Purpose

Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pICE-NLS-mCherry

Plasmid

#60364

Purpose

Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

NLS-mCherry

Tags

NLS from SV40 T antigen and mCherry

Promoter

CMV-tet

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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FusionRed-Lifeact-7

Plasmid

#54778

Purpose

Localization: Actin, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Lifeact-FusionRed

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pHLUM

Plasmid

#40276

Depositor

Markus Ralser

Article

Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

Inserts

LEU2 (LEU2 Budding Yeast)
URA3 (URA3 Budding Yeast)
MET15/17 (MET17 Budding Yeast)

Expression

Yeast

Promoter

endogenous LEU2, endogenous MET15/17, and endogen…

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pOPINE

Plasmid

#26043

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His-lacZ-KHHHHHH

Tags

KHHHHHH and MAHHHHHHSSG

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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TAp63gamma-(3X)HA

Plasmid

#26977

Depositor

David Sidransky

Article

Upadhyay et al Biochem Biophys Res Commun. 2007 May 18.

Insert

TAp63gamma (TP63 Human)

Tags

3XHA

Expression

Mammalian

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+FFluc

Plasmid

#26156

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Firefly luciferase

Expression

Bacterial

Mutation

Firefly luciferase gene codon optimised for mycob…

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pSO2002

Plasmid

#25842

Depositor

Aziz Sancar

Article

Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-3

Insert

human Cryptochrome 2 (CRY2 Human)

Tags

flag, his, and myc

Expression

Mammalian

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKACA

Plasmid

#23495

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACA (PRKACA Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCEP4-tat

Plasmid

#22502

Depositor

Sergey Kasparov

Article

Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-

Insert

Transactivator of Transcription

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pMyrPalm_mEGFP

Plasmid

#21037

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Tags

mEPFP

Expression

Bacterial and Mammalian

Available Since

May 12, 2009

Availability

Academic Institutions and Nonprofits only

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PGEX3X-GST-PTEN

Plasmid

#20743

Depositor

Alonzo Ross

Article

Campbell et al J Biol Chem. 2003 Sep 5. 278(36):33617-2

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only
pRSET FlipSuc-90u delta1

Plasmid

#14475

Depositor

Wolf Frommer

Article

Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

Insert

FlipThuE W283A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

N192D, W283A, 13 amino acids deleted from linkers

Promoter

T7

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-Tcl1

Plasmid

#13364

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

T-cell lymphoma breakpoint 1 (Tcl1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-Utf1

Plasmid

#13369

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

undifferentiated embryonic cell transcription factor 1 (Utf1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-AML1-ETO

Plasmid

#12428

Depositor

Scott Hiebert

Article

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Insert

AML1-ETO fusion (RUNX1, RUNX1T1 Human)

Expression

Mammalian

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only

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