We narrowed to 23,573 results for: CHI

1. NET5s pEGFP-N2 (1154)

   Plasmid

   #62034

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET5s (TMEM201 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   short form

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. EGFP-hPMCA2x/b

   Plasmid

   #47585

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   pMCA2x/b (ATP2B2 Human)
   

   Use

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTXB1-H2B(1-114)_wt

   Plasmid

   #118252

   Purpose

   Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   xlH2B(1-114)
   

   Use

   Tags

   Mxe-GyrA intein fused to chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLINE1-scFvFc

   Plasmid

   #206020

   Purpose

   An intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGIPz-mPD-L1 3SA

   Plasmid

   #121489

   Purpose

   Expression of mouse PD-L1 3SA

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1 3SA
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S175A, T179A, S183A

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-hPMCA2z/b

   Plasmid

   #47584

   Depositor

   Emanuel Strehler

   Article

   Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

   Insert

   pMCA2z/b (ATP2B2 Human)
   

   Use

   Tags

   EGFP-HA-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

8. LentiCRISPRv2puro-Tbxas1

   Plasmid

   #170305

   Purpose

   A knock-out vector for the mouse Tbxas1

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Tbxas1 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-dLight1.3a

   Plasmid

   #111055

   Purpose

   Expresses dLight1.3a in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   dLight1.3a
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBa.GFP-AP1/beta1

    Plasmid

    #218802

    Purpose

    Mammalian expression o fhuman AP1 b1

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    AP1 b1 (AP1B1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB.FRB-3myc-KLC1a

    Plasmid

    #134627

    Purpose

    Mammalian expression of mouse KLC1a

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KLC1a (Klc1 Mouse)
    

    Use

    Tags

    FRB, 3myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro

    Plasmid

    #192683

    Purpose

    Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDule-IBBN (G2)

    Plasmid

    #85500

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6/V5-DEST-UCHL5

    Plasmid

    #31219

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    UCHL5 (UCHL5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pGIPz-PD-L1 3SA

    Plasmid

    #121490

    Purpose

    Expression of human PD-L1 3SA with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Inserts

    PD-L1 3SA
    humun PD-L1 shRNA
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S176A, T180A, S184A

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR P4-P1R-mKate2

    Plasmid

    #48344

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    It contains a Kozak sequence but no stop codon.

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. WFS1 pEGFP-N2 (1472)

    Plasmid

    #62051

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    WFS1 (WFS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLenti6/V5-DEST-NDC80

    Plasmid

    #31215

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    NDC80 (NDC80 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

19. LentiCRISPRv2puro-Tbxa2r

    Plasmid

    #170304

    Purpose

    A knock-out vector for the mouse Tbxa2r

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxa2r gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH-PD-L1 3SA/4NQ

    Plasmid

    #121491

    Purpose

    Expression of human PD-L1 3SA/4NQ with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET32a-Trx-PP-hNF2

    Plasmid

    #107148

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

    Insert

    neurofibromin 2 (NF2 Human)
    

    Use

    Tags

    (His)6-Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS0035

    Plasmid

    #131125

    Purpose

    Mammalian Target-AID expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1 and UGI

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCA-T-intron(Neo)-G(LNL)

    Plasmid

    #36908

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p7612 MSCV-P C-FlagHA 16E7 E10K

    Plasmid

    #163303

    Purpose

    Expresses HPV16 E7 E10K

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 E10K
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    E10K

    Promoter

    MSCV LTR

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR221 NLRP1 S1213A

    Plasmid

    #166824

    Purpose

    Gateway pDONR vector containing NLRP1 with the P1214R patient mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    S1213A

    Promoter

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. NET5L pEGFP-N2 (1155)

    Plasmid

    #62035

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    NET5L (TMEM201 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    long form

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

27. POPDC2 pEGFP-N2 (1087)

    Plasmid

    #62030

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    POPDC2 (POPDC2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

28. NET30 pEGFP-N2 (815)

    Plasmid

    #62011

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET30 (MOSPD3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pOTTC1617 pAAV SYN1 Nuc-EYFP

    Plasmid

    #135567

    Purpose

    An AAV vector expressing a neuronally expressed nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    empty
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN1

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTol2CG2-QUASM1:mScarletNLS-SV40pA

    Plasmid

    #155124

    Purpose

    Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QUASM1:mScarletNLS
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    QUASM1-E1b

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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