We narrowed to 23,602 results for: CRISPR

Showing: 3261 - 3280 of 23602 results

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pTU422

Plasmid

#178982

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Inserts

gRAMP
CRISPR array

Tags

Strep-Tag II SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDuRCC-N

Plasmid

#81194

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please view depositor comments below and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-CjCAS9-pA

Plasmid

#131543

Purpose

Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

Insert

CjCas9

Use

CRISPR

Tags

FLAG-SV40NLS, SV40NLS, and polyA

Expression

Mammalian

Mutation

Human codon-optimized

Promoter

CAG, T3

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-UGCG-KO

Plasmid

#80010

Purpose

gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

UGCG (UGCG Human)

Use

CRISPR

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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sg14x(MS2) MUC4.1

Plasmid

#101153

Purpose

gRNA with 14 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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MASTL B4.4 gRNA

Plasmid

#90755

Purpose

3rd generation lentiviral gRNA plasmid targeting human MASTL

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MASTL (Guide Designation B4.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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A3Bctd-max

Plasmid

#198888

Purpose

Express A3Bctd-max BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Tags

6x His and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA TdR

Plasmid

#80940

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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KIF20A D5.2 gRNA

Plasmid

#90718

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF20A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF20A (Guide Designation D5.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-GFP(1-10)

Plasmid

#224566

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and split GFP(1-10) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-GFP(1-10)

Use

CRISPR

Tags

T2A-split GFP(1-10)

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMK-Cas9-gate

Plasmid

#113743

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGE_En01

Plasmid

#71534

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Ikuko Hara-Nishimura, Takayuki Kohchi

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9-VRER_2A_GFP

Plasmid

#75475

Purpose

Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

Depositor

Marc Tessier-Lavigne

Article

Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

Insert

Cas9-VRER

Use

CRISPR

Tags

2A_GFP

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

CAG

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only

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FEN1 C10.3 gRNA

Plasmid

#90689

Purpose

3rd generation lentiviral gRNA plasmid targeting human FEN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FEN1 (Guide Designation C10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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SMC6 G4.4 gRNA

Plasmid

#90899

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC6 (Guide Designation G4.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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SMC6 G3.4 gRNA

Plasmid

#90898

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC6

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC6 (Guide Designation G3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJG80

Plasmid

#89282

Purpose

Entry vector containing TaCas9-nos terminator between attL1 and attR5 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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BRCA1 A9.2 gRNA

Plasmid

#90548

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A9.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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