We narrowed to 21,642 results for: ESC

1. pIG-727_HA-GD2-28z_CAR_RFP-tNGFR

   Plasmid

   #207487

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP-tNGFR, HA-GD2-28z_CAR (NGFR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-2xFLAG-2xSTREP-CDK6

   Plasmid

   #172614

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK6 (CDK6 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-FLEx-iSeroSnFR

   Plasmid

   #128486

   Purpose

   Fluorescent reporter for serotonin (conditional + membrane localization tag)

   Depositor

   Lin Tian

   Article

   Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

   Has Service

   AAV9

   Insert

   iSeroSnFR
   

   Use

   AAV

   Tags

   Ig-kappa leader, Myc, PDGFR, and cpsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV ArcLightCo (Q239)-T2A-nls-mCherry

   Plasmid

   #85806

   Purpose

   Genetically encoded voltage sensor ArcLight- codon optimized

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   ArcLightCo-Q239
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

   Plasmid

   #172618

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr283Ala

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-FLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172619

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. px458-CCND1

   Plasmid

   #172630

   Purpose

   Expresses a gRNA against CCND1 and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   gRNA against CCND1 (CCND1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKII-Archon1-KGC-EGFP-ER2

   Plasmid

   #108419

   Purpose

   AAV production plasmid encoding for Archon1 fluorescent voltage reporter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. shERK2b-mlpx-puro

   Plasmid

   #65230

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

    Plasmid

    #172647

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-FLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172626

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherry

    Plasmid

    #172639

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIG-836_NY-ESO-1_TCR_FAS/OX40

    Plasmid

    #207499

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/OX40, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. shERK2a-mlpx-puro

    Plasmid

    #65229

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIG-833_NY-ESO-1_TCR_FAS/CD28

    Plasmid

    #207496

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/CD28, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-Archon2-KGC-EGFP-ER2

    Plasmid

    #108421

    Purpose

    AAV production plasmid encoding for Archon2 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon2-KGC-EGFP-ER2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIG-671_HA-GD2-28z_CAR_tNGFR

    Plasmid

    #207484

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIG-874_HA-GD2-28z_CAR_IL2RA

    Plasmid

    #207504

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    IL2RA, HA-GD2-28z_CAR (IL2RA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

    Plasmid

    #172609

    Purpose

    Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    2xFLAG-mAID-AMBRA1 (AMBRA1 Synthetic, Human)
    

    Use

    CRISPR; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVZ_PcoaT_SQE1_LUP1

    Plasmid

    #103857

    Purpose

    Heterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803

    Depositor

    Ilka Axmann

    Article

    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d

    Insert

    lupeol synthase 1 (LUP1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    inserted genes are codon optimized. 2nd amino aci…

    Promoter

    PcoaT (from Synechocystis sp. PCC 6803)

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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