Purpose
The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
Inserts
cas9
pyrG
U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
Use
CRISPR; Fungal expression
Tags
SV40 NLS
Expression
Bacterial
Mutation
Available since
May 11, 2018
Availability
Academic Institutions and Nonprofits only