We narrowed to 73,945 results for: LAS

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pC4-RhE-FRB-Fis1

Plasmid

#68056

Purpose

express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

Fis1 (FIS1 Human)

Tags

FRB

Expression

Mammalian

Mutation

Fis1 tail (aa 93-152)

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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hWIP1 FLAG

Plasmid

#28105

Depositor

Lawrence Donehower

Article

Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

Insert

WIP1 (PPM1D Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pBMN-2xFKBP-mEGFP-NDP52

Plasmid

#110465

Purpose

retrovirus construct for stable expressing mEGFP tagged NDP52

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral; Retrovirus

Tags

mEGFP

Expression

Mammalian

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5-K130R

Plasmid

#13096

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5-K130R (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

K130R

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pBA407

Plasmid

#85970

Purpose

UPRE-mCh-Ubc-Neo

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

mCherry
Neo

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pNS35 (multiAsCas12a piggyBac)

Plasmid

#217336

Purpose

piggyBac expression of multiAsCas12a

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-P2A-TagBFP2

Expression

Mammalian

Mutation

R1226A/E174R/S542R/K548R

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSLIK-Zeo

Plasmid

#25736

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)

Use

AAV and Cre/Lox

Mutation

TVA mammalian codon optimized

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-CIBN-CreC

Plasmid

#26889

Purpose

Expresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN-CreC

Expression

Mammalian

Mutation

aa 1-170 of CIB1, aa 106-343 of Cre

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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CD8-mCherry-FRB

Plasmid

#100738

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Tags

FRB and mCherry

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAV126

Plasmid

#63192

Purpose

pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCa-C1

Plasmid

#112264

Purpose

ITRACKalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

Beta-actin

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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FKBP-epsin(144-575)-GFP

Plasmid

#100733

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

epn1 (Epn1 Mouse)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

clathrin binding middle part aa 144-575

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pROSA26-promoter

Plasmid

#21710

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 promoter (Gt(ROSA)26Sor Mouse)

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pX335-EN475

Plasmid

#86231

Purpose

For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse CTCF STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pX335-EN477

Plasmid

#86232

Purpose

For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse CTCF STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAV116

Plasmid

#63183

Purpose

pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAV125

Plasmid

#63191

Purpose

pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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