We narrowed to 13,780 results for: TIM

1. pUC19-Hph

   Plasmid

   #188741

   Purpose

   pUC19 plasmid (MCS intact) with Hygromycin selection marker

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   Hygromycin
   

   Use

   Filamentous fungal expression

   Tags

   Expression

   Mutation

   Promoter

   gpdA

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS-H2B-cerulean

   Plasmid

   #53748

   Purpose

   Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   Cerulean

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9 MDC123

   Plasmid

   #59184

   Purpose

   2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

   Depositor

   Robert Stupar

   Article

   Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

   Insert

   Glycine Max codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2X35S

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_763

   Plasmid

   #201156

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D908A

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS-H2B-mRFP1

   Plasmid

   #53745

   Purpose

   Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. CIB1-CreC(N1)

   Plasmid

   #75367

   Purpose

   Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CIB1 (CIB1 Mustard Weed)
   

   Use

   Tags

   Cre DNA recombinase, residues 106-343

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. RT3GEPIR

   Plasmid

   #111169

   Purpose

   Tet-regulated miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   T3G

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-ChroME-mRuby2-ST

   Plasmid

   #108902

   Purpose

   Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   ChroME-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-Nup35-mut

   Plasmid

   #87341

   Purpose

   To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP35 (NUP35 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-Electra2-P2A-EGFP

    Plasmid

    #184936

    Purpose

    Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. tetO-NKX2.5

    Plasmid

    #46029

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Insert

    NKX2.5 (NKX2-5 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-LgBiT-SpyCatcher

    Plasmid

    #223634

    Purpose

    Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6, LgBiT

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

    Plasmid

    #191457

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCSF-R-luc

    Plasmid

    #12420

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

15. mt deltaN20 TFB2MdeltaC3

    Plasmid

    #205062

    Purpose

    TFB2M protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    TFB2M (TFB2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N-terminal 20 aa and C-terminal 3 aa

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    GAL4-responsive UAS hsp70

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-MSP1D1delltaH4

    Plasmid

    #71713

    Purpose

    Membrane scaffold protein (MSP), helix 4 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2/Capsid

    Plasmid

    #120816

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    Capsid ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Stx4-pmCitrine

    Plasmid

    #92422

    Purpose

    Expression of syntaxin-4 C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CMV-eGFP-SV40p-BlasR

    Plasmid

    #228271

    Purpose

    pLenti-CMV-eGFP-SV40p-BlasR plasmid for ectopic expression of GFP

    Depositor

    Judy Lieberman

    Article

    Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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